PrestoBlue™ Cell Viability Reagent
PrestoBlue™ Cell Viability Reagent
Invitrogen™

PrestoBlue™ Cell Viability Reagent

Get high quality cell viability results and instant time savings with PrestoBlue® Cell Viability Reagent. PrestoBlue® is a ready toRead more
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Catalog NumberQuantity
A13262100 mL
A1326125 mL
Catalog number A13262
Price (USD)
692.00
Each
Add to cart
Quantity:
100 mL
Price (USD)
692.00
Each
Add to cart
Get high quality cell viability results and instant time savings with PrestoBlue® Cell Viability Reagent. PrestoBlue® is a ready to use cell permeable resazurin-based solution that functions as a cell viability indicator by using the reducing power of living cells to quantitatively measure the proliferation of cells. When added to cells, the PrestoBlue® reagent is modified by the reducing environment of the viable cell and turns red in color, becoming highly fluorescent. This color change can be detected using fluorescence or absorbance measurements.

The PrestoBlue® Cell Viability Reagent allows you to:

• Save time with an incubation step as short as 10 minutes.
• Obtain high quality results with a large dynamic range
• Experience convenience with the add and read homogenous format
• Monitor living cells and perform downstream functional assays

10-minute incubations
The PrestoBlue® Cell Viability Reagent is the only live-cell viability reagent on the market able to provide robust data typically in as little as 10 minutes following reagent addition. All other resazurin-based cell viability reagents on the market require a one to four hour incubation with the reagent prior to obtaining data.

High quality results
Based on the cell type and incubation time, PrestoBlue® Cell Viability Reagent can detect as few as 12 cells per well in a 384 well plate. After ten minutes of incubation the PrestoBlue® Reagent can linearly detect cells down to 98 cells⁄well with a Z’ value of greater than 0.5.

Homogenous assay format
The PrestoBlue® Cell Viability Reagent is an addition-only reagent. Without the cell lysis or solubilization steps required by other assays on the market, hands-on time is minimized.

Live cell assays
The PrestoBlue® Cell Viability Reagent does not require cell lysis. Cells may be incubated for up to 24 hours in the presence of PrestoBlue® Cell Viability Product. Simply remove the reagent from the cells and replace it with growth medium for further proliferation.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeBacteria, Eukaryotic Cells, Fungal Cells
ColorBlue
Concentration10 X
DescriptionPrestoBlue Cell Viability Reagent
Detection MethodColorimetric, Fluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Microplate Reader, Spectrophotometer, HTS Reader
Product TypeReagent
Dye TypeResazurin
Emission590-615 nm
Excitation Wavelength Range535-560 nm
FormLiquid
Format96-well plate, Cuvettes, 384-well plate
Incubation Time10 min. to 2 hr.
Product LinePrestoBlue
Quantity100 mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
ThroughputHigh-throughput Compatible
Unit SizeEach
Contents & Storage
Store at 4°C and protect from light.

Frequently asked questions (FAQs)

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why would I use PrestoBlue HS Cell Viability Reagent instead of the original PrestoBlue Cell Viability Reagent?

PrestoBlue HS Cell Viability Reagent provides a lower background, suitable for analyzing a lower number of cells per well. Also, the removal of impurities in the 'HS' reagent limits any artifacts that may be critical to your cells' viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does PrestoBlue HS Cell Viability Reagent have the same properties as the original PrestoBlue Cell Viability Reagent?

Yes and in addition, PrestoBlue HS Cell Viability Reagent has a higher purity which results in a lower background.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between PrestoBlue HS Cell Viability Reagent (Cat. Nos. P50200, P50201) and the original PrestoBlue Cell Viability Reagent (Cat. Nos. A13261, A13262)?

Both products utilize resazurin as the indicator dye. PrestoBlue HS Cell Viability Reagent undergoes a purification process that removes any residual resorufin that may be present from the standard manufacturing process for resazurin. This purification results in a product that has very low background. PrestoBlue HS Cell Viability Reagent may be used on samples with as few as 10 cells/well, compared to a minimum of 12 cells/well for the orginal PrestoBlue Cell Viability Reagent.
Note: Minimum cell number/well is also dependent upon reducing activity of the cell type. Results may vary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.