MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?
This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?
This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Why would I use PrestoBlue HS Cell Viability Reagent instead of the original PrestoBlue Cell Viability Reagent?
PrestoBlue HS Cell Viability Reagent provides a lower background, suitable for analyzing a lower number of cells per well. Also, the removal of impurities in the 'HS' reagent limits any artifacts that may be critical to your cells' viability.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Does PrestoBlue HS Cell Viability Reagent have the same properties as the original PrestoBlue Cell Viability Reagent?
Yes and in addition, PrestoBlue HS Cell Viability Reagent has a higher purity which results in a lower background.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
What is the difference between PrestoBlue HS Cell Viability Reagent (Cat. Nos. P50200, P50201) and the original PrestoBlue Cell Viability Reagent (Cat. Nos. A13261, A13262)?
Both products utilize resazurin as the indicator dye. PrestoBlue HS Cell Viability Reagent undergoes a purification process that removes any residual resorufin that may be present from the standard manufacturing process for resazurin. This purification results in a product that has very low background. PrestoBlue HS Cell Viability Reagent may be used on samples with as few as 10 cells/well, compared to a minimum of 12 cells/well for the orginal PrestoBlue Cell Viability Reagent.
Note: Minimum cell number/well is also dependent upon reducing activity of the cell type. Results may vary.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.