Hibernate™-E Medium
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Hibernate™-E Medium
Gibco™

Hibernate™-E Medium

Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium isRead more
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Catalog number A1247601
Price (USD)
167.00
Each
Add to cart
Price (USD)
167.00
Each
Add to cart
Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is optimized for embryonic cells or tissue in ambient carbon dioxide levels.

Hibernate Medium enables neurons to be maintained in ambient CO2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

Hibernate Medium may be combined with B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of Hibernate Medium and B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Stem Cells
ClassificationSerum-free
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeStem Cell Media
Serum LevelSerum-free
Shelf Life24 Months
SterilitySterile
With AdditivesPhenol Red
Product LineHibernate™
Quantity500 mL
Shipping ConditionAmbient
Tested ForCytotoxicity, Endotoxin, Sterility, pH, Osmolality
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Can the Hibernate-E Medium (Cat. No. A1247601) be used for plating and growing mouse primary hippocampal neurons?

Hibernate-E medium (Cat. No. A1247601) is designed for the maintenance of embryonic neural tissue and is suitable for short-term maintenance of cultured rat neurons at 37°C in ambient CO2 (up to 2 days) and long-term storage of viable brain tissue at 2 to 8°C (up to a month). However, it is not recommended for the initial plating and growing of neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What medium can be used to maintain/manipulate neurons at ambient CO2?

We offer Gibco Hibernate Medium for this purpose. When supplemented with Gibco B-27 Supplement and Gibco GlutaMAX-1 Supplement, this medium can allow for the manipulation of neurons at ambient CO2 for at least 48 hours while retaining their viability, and preserve viable brain tissue for up to a month when stored at 4 degrees C. Two Hibernate media are offered:

- Gibco Hibernate-A Medium (Cat. No. A1247501) is formulated for postnatal neurons.
- Gibco Hibernate-E Medium (Cat. No. A1247601) is formulated for embryonic neurons.

These two media have the same formulation and differ only in osmolality.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.