Alexa Fluor™ 488 Phalloidin
Alexa Fluor™ 488 Phalloidin
Invitrogen™

Alexa Fluor™ 488 Phalloidin

Alexa Fluor 488 phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488Read more
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Catalog number A12379
Price (USD)
571.65
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618.00
Save 46.35 (8%)
Each
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Price (USD)
571.65
Online Exclusive
618.00
Save 46.35 (8%)
Each
Add to cart
Alexa Fluor 488 phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye.

Also available as a room-temperature-stable, ready-to-use solution: ActinGreen 488 Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 495/518 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples

Get Superior Results in Your Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor 488 provides green fluorescence of unparalleled brightness and photostability.

Use in Multiple Applications
Alexa Fluor 488 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor 488 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals and other Alexa Fluor conjugates including secondary antibodies.

Read about labeling F-Actin with phallotoxins

Find out more about our Alexa Fluor 488 products

Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool

See the full line of Alexa Fluor Dye products

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation Wavelength Range495/518 nm
Dye TypeAlexa Fluor™ 488
Quantity300 Units
Product LineAlexa Fluor™
Shipping ConditionRoom Temperature
Label TypeAlexa Fluor Dyes
Product TypePhalloidin
SubCellular LocalizationActin, Cytoskeleton
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.