Competent Cell Sampler

The Competent Cell Sampler Kit contains a pair of four different strains of chemically competent E. coli cells in One Shot format. Each single-use vial of cells is ready to use, with no extra pipetting steps or freeze-thaw cycles that reduce transformation efficiency. Use this kit to evaluate which strains best suit the different types of DNA used in your lab.

One Shot Mach1 T1 Chemically Competent E. coli
One Shot Mach1 T1 Chemically Competent E. coli cells are phage resistant and have a faster doubling time (∼50 minutes) than other popular E. coli cells. As a result, Mach1-T1^R colonies are clearly visible within eight hours of plating transformation on ampicillin selection plates. From an overnight colony, minipreps can be performed after four hours of growth. The Mach1-T1^R strain is derived from a non-K-12, wild-type W E. coli strain that has high growth speed and has been genetically manipulated to possess the most useful cloning strain features.

Mach1-T1^R competent E. coli cells come in One Shot format (Cat. No. C862003) or MultiShot StripWell and FlexPlate formats for high throughput applications (Cat. No. C869601 and C8681201).

One Shot OmniMAX2 T1^R Chemically Competent E. coli
The One Shot OmniMAX2 T1^R Chemically Competent E. coli strain is a versatile phage-resistant strain, perfect to use in all cloning applications, including Gateway technology, TOPO PCR cloning, and cDNA and gDNA library construction. It offers the highest transformation efficiency in a chemically competent One Shot format with 5 x 10⁹ transformants/μg plasmid DNA. The ccdA gene present on the F´ episome, carried by OmniMAX 2-T1^R, is deleted, allowing negative selection of Gateway vectors since the strain is sensitive to the ccdB gene product.

OmniMAX 2-T1^R cells are offered in our One Shot format (Cat. No. C854003) or MultiShot FlexPlate format for high throughput applications (Cat. No. C8581201).

One Shot Stbl3 Chemically Competent E. coli
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral or retroviral vectors. Stbl3 had been essential for many applications, including genome editing CRISPR workflows or vaccine development. The DNA yield from Stbl3 cells is often >10-fold higher than standard cloning strains.

Stbl3 chemically competent cells are available in One Shot format (Cat. No. C737303) or MultiShot StripWell and FlexPlate formats (Cat. No. C739601 and C7381201) for high throughput applications.

One Shot TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are one of the most popular competent cell strains and are perfect for a broad range of cloning applications. They are included with many of our cloning and expression kits like TOPO and Gateway cloning kits and complement our GeneArt Gene Synthesis Services.

One Shot TOP10 competent E. coli have a transformation efficiency of 1 x 10⁹ cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids.

TOP10 chemically competent cells are available in the One Shot format (Cat. No. C404003) or MultiShot StripWell and FlexPlate formats (Cat. No. C409601 and C4081201) for high throughput applications. TOP10 cells are also available for transformation using electroporation (Cat. No. C404050).