AbC™ Anti-Mouse Bead Kit
AbC™ Anti-Mouse Bead Kit
Invitrogen™

AbC™ Anti-Mouse Bead Kit

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometryRead more
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Catalog number A10344
Price (USD)
398.00
Each
Add to cart
Price (USD)
398.00
Each
Add to cart
The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.

• Species reactivity—for use with all isotypes of mouse antibody conjugates
• Maximum laser compatibility—for use with all lasers

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Mouse Bead Kit was developed to address this issue.

View a selection guide for all flow cytometry compensation beads.

How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.

Species reactivity
The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins.

Maximum laser compatibility
All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Diameter (Metric)6 μm
Excitation Wavelength Rangeany lasers (UV to 633/635)
For Use With (Equipment)Flow Cytometer
FormatTube
No. of Tests100 tests
Quantity1 kit
Shipping ConditionRoom Temperature
Stain ConfigurationSurface
Product LineCompenFlow™, Molecular Probes™
Product TypeCompensation Beads
Unit SizeEach
Contents & Storage
Contains 1 vial AbC™ capture beads and 1 vial of negative beads.
  • Store at 2°C to 8°C

  • Frequently asked questions (FAQs)

    I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?

    It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    I’ve been told that I need to compensate my data for flow cytometry analysis. What does that mean?

    By running single-color controls, it is possible to remove signal of one fluorophore that spills over into the collection channel for another fluorophore.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    What kind of controls do I need for flow cytometry?

    For compensation, you need to prepare a singly stained sample (or compensation beads) for each color parameter that you are using. In addition, we recommend that you use FMO (flow minus one) controls. These are controls in which you label cells or beads with every color in your panel, omitting one. Make one FMO control for each color. These controls are important for helping you properly set gates on your data.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Why should I worry about compensation in flow cytometry analysis?

    In a perfect world, the fluorescence emission profile for each individual fluorophore would be a very intense, narrow peak, well separated from all other emission peaks. In reality, organic dyes and fluorescent proteins have broad emission peaks, and compensation must be employed (during or after data acquisition) to correctly assign fluorescence signal to each fluorophore. Compensation is important because it removes fluorescent signal from overlapping spectra so you know that the signal you see is only the signal from the fluorophore of interest.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    How do I make compensation controls for antibodies?

    All that you need for compensation controls is an unstained sample of your cells (or negative control beads) and samples with each of your fluorophores - one per tube. You want the single-stained controls to be as bright as you expect your brightest sample to be. Antibodies can be bound to beads instead of cells using our AbC Total Antibody Compensation Bead Kit (Cat. Nos. A10513 and A10497).

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.