CELLstart™ Substrate
CELLstart™ Substrate
Gibco™

CELLstart™ Substrate

CELLstart™ is a defined substrate, containing only components of human origin (xeno-free).•Defined, xenofree substrate for stem cell culture•cGMP Manufactured toRead more
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Catalog number A1014201
Price (USD)
403.00
Each
Add to cart
Price (USD)
403.00
Each
Add to cart
CELLstart™ is a defined substrate, containing only components of human origin (xeno-free).•Defined, xenofree substrate for stem cell culture
•cGMP Manufactured to help ensure manufacturability and traceability

CELLstart™ is a defined substrate, containing only components of human origin (xeno-free). CELLstart™ supports human embryonic stem cell (hESC) attachment and expansion of undifferentiated colonies in serum-free medium without the need for feeder cells. CELLstart™ can also be used with mesenchymal stem cells, neural stem cells, and for the attachment of human feeder cells (e.g. foreskin fibroblasts).

GIBCO™ products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis and Certificates of Origin.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells (Neural), Stem Cells (iPS - Induced Pluripotent Stem), Stem Cells (Embryonic), Stem Cells (Mesenchymal)
ClassificationAnimal Origin-Free, Serum-Free, Xeno-Free
FormLiquid
Product LineGibco™
Quantity2 mL
Endotoxin Level≤50 EU/mL, ≤ 50 EU/mL
FormatTube(s)
Phenol Red IndicatorNo Phenol Red
Purity or Quality GradehESC-Qualified
Shipping ConditionRoom Temperature
SterilitySterile
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C.

Frequently asked questions (FAQs)

How do I prepare the StemPRO hESC SFM complete medium?

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax matrix, 2 ml StemPRO hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can StemPRO hESC SFM be used to culture mouse ESCs?

No, we don't recommend StemPro hESC SFM for mouse cells because they showed poor growth and some differentiation. We recommend KnockOut DMEM and KnockOut SR for mouse ESC cultures (catalog numbers 10829-018 and 10828-028 respectively).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can StemPRO hESC SFM be used for new cell line derivations?

Yes, derivations using StemPro hESC SFM have been carried out using either a basement membrane extract (like Geltrex growth factor) or feeder cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell lines has StemPRO hESC SFM been tested with?

StemPro hESC SFM has been tested with the following hESC: H1, H9, BG01, BG02, BG03, HUES6, HUES9, Cyth203, CyT49, BG01v, HES-2, HES-3, KhES-1, and TE06. The medium has also been used with the Rhesus monkey line R336.4. Some cells may require a short adaptation time but most do not.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does StemPRO hESC SFM require the use of a basement membrane matrix?

Yes, we recommend using Geltrex hESC-qualified Reduced Growth Factor basement membrane (catalog number A10480-02), CELLstart (catalog number A10142-01) or similar matrix.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all CELLstart™ Substrate FAQs