StemPro™ Chondrogenesis Differentiation Kit
StemPro™ Chondrogenesis Differentiation Kit
Gibco™

StemPro™ Chondrogenesis Differentiation Kit

The StemPro® Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels.Read more
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Catalog number A1007101
Price (USD)
252.00
Each
Add to cart
Price (USD)
252.00
Each
Add to cart
The StemPro® Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the chondrogenesis pathway and generate chondrocytes. Using StemPro® Chondrogenesis Differentiation Kit in combination with StemPro® MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion, and differentiation into collagen matrix-producing chondrocytes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeStem Cells (Mesenchymal)
Culture EnvironmentCO2
For Use With (Application)Stem Cell Differentiation
Product TypeChondrogenesis Differentiation Kit
Endotoxin Level≤50 EU/mL, ≤ 50 EU/ml
FormatLiquid
GlucoseLow Glucose
GlutamineGlutaMAX™-I
HEPES BufferNo HEPES
Phenol Red IndicatorNo Phenol Red
Product LineGibco™, StemPro™
Purity or Quality GradeResearch Grade
Quantity1 Kit
Research CategoryDifferentiation
Serum LevelSerum-Free
Sodium Bicarbonate BufferSodium Bicarbonate
Sodium Pyruvate AdditiveSodium Pyruvate
SpeciesHuman
Unit SizeEach
Contents & Storage
This kit is shipped with a 100 ml basal medium and 10 ml growth supplement adequate to formulate 100 ml of complete medium. Components require separate shipping and storage requirements. The growth supplement is not sold individually.
StemPro™ Osteocyte⁄Chondrocyte Differentiation Basal Medium 2 to 8°C (protect from light)
StemPro™ Chondrogenesis Supplement -5 to -20°C (in the dark)

Frequently asked questions (FAQs)

How many times can I freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A1006401) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

We do not recommend that you freeze/thaw the StemPro Chondrogenesis Supplement (Cat. No. A10064-01) from the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101). Addition of supplement to the StemPro Osteocyte/Chondrocyte Differentiation Basal Medium (Cat. No. A1006901) is stable at 2-8 degrees C for up to 1 month, protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What mesenchymal stem cells (MSC) sources can I use with the StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101)?

The StemPro Chondrogenesis Differentiation Kit (Cat. No. A1007101) was designed and tested using human adipose-derived stem cells (ADSC). Based on protocols in literature, it should also work with mesenchymal stem cells (MSC) from other sources, including umbilical cord and cord blood.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The StemPro Chondrogenesis Differentiation Kit manual refers to MSC Attachment Medium under Chondrogenesis Differentiation, Step 7. Is MSC Attachment Medium the same as MSC growth medium?

Yes, MSC Attachment Medium is the same as MSC growth medium.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

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View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

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