Axiom™ Microbiome, 96 array plate
Axiom™ Microbiome, 96 array plate
Applied Biosystems™

Axiom™ Microbiome, 96 array plate

Easy, streamlined solution for microbial profilingAxiom Microbiome Array enables the detection of all known microorganisms in a sample with species-Read more
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Catalog number 902904
Price (USD)
9,980.00
Each
Add to cart
Price (USD)
9,980.00
Each
Add to cart
Easy, streamlined solution for microbial profiling
Axiom Microbiome Array enables the detection of all known microorganisms in a sample with species- and strain-level identification in a single, scalable assay. Utilizing the robust biochemistry of Axiom 2.0 Assay coupled with manual and automated target preparation methods, this streamlined protocol provides consistent and high-quality results. A simplified, upstream reverse transcriptase reaction enables the detection of RNA virus genomes. Hands-free array processing is performed on GeneTitan Multi-Channel Instrument. The complete solution includes arrays, reagent kits, and free, easy-to-use microbiome analysis software (see Figures 1 and 2).

Highlights:
• Expert design for profiling human and animal microbiomes with a single assay
•Detects greater diversity and higher resolution than 16s rRNA gene sequencing
•Allows genetic profiling of all organisms present in a sample
•Comprehensive coverage of over 11,000 organisms and five microbial domains: archaea, bacteria, fungi, protozoa, and viruses
• Allows identification to the species, strain and sequence level.
•Flexible 24- and 96-array plate formats
•Sample type agnostic: applications in nutrigenomics, agrigenomics, and animal research
•Simplified microbial detection analysis

See also Axiom Microbiome, 24 array plate and Axiom Microbial Detection Analysis Software (MiDAS).

See Figure 2 for target categories represented on Axiom Microbiome Arrays.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ArrayGenotyping
Number of Arrays96 arrays
Format96-array Plate
SpeciesProtozoa, Archaea, Bacteria, Fungi, Virus
For Use With (Application)Microarray Analysis
Quantity1 plate
Unit SizeEach

Frequently asked questions (FAQs)

Is the hybridization mix for cartridges the same as the hybridization mix for PEG arrays (array plates)?

The hybridization mix for PEG arrays is different from the hybridization mix for cartridges. The concentrations of the hybridization mix are different and in addition, DMSO is not added into the hybridization mix for PEG arrays because it can affect the glue that holds the PEG to the plate.

What is the starting amount of DNA required for Axiom arrays?

All Axiom arrays (except for Axiom Genome-Wide Pan-African Array Set) require a total of 200 ng DNA. The Axiom Genome-Wide Pan-African Array Set requires a total of 300 ng DNA, or 100 ng DNA per array (there are 3 arrays).

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What are the sample requirements for Axiom array processing at the Thermo Fisher Scientific Microarray Research Services Laboratory (MRSL)?

For information regarding our in-house MRSL requirements, please contact us via phone at 888-362-2447 (in North America) or via email at BioinformaticsServices@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What sources of genomic DNA (gDNA) have been tested to use with the Axiom Microbiome Array (Cat. Nos. 902904, 902903)?

gDNA extracted from stool samples has been tested for use with the Axiom Microbiome Array. DNA derived from FFPE (formalin-fixed paraffin-embedded) blocks should not be used with this assay.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What was the criteria for selecting covered species on the Axiom Microbiome Array?

The species included on the array represent all known microbial sequences available in the NCBI database as of October 2014 for which probes could be designed. Any sequences deposited after October 2014 are not represented on the array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.