Pierce™ Glycoprotein Isolation Kit, WGA
Pierce™ Glycoprotein Isolation Kit, WGA
Thermo Scientific™

Pierce™ Glycoprotein Isolation Kit, WGA

The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissueRead more
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Catalog number 89805
Price (USD)
499.00
Each
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Price (USD)
499.00
Each
Add to cart
The Thermo Scientific Pierce Glycoprotein Isolation Kit (Wheat Germ Agglutinin) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing N-Acetyl glucosamine (GlcNAc) groups or sialic acid. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Features of this Glycoprotein Isolation Kit:

• High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins
• Fast—glycoprotein purification in less than an hour
• Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate.
• Robust—lectin does not leach from resin when processing sample
• Convenient—complete kit offering lectin resins and spin columns with all necessary reagents
• Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Related Products
Pierce™ Glycoprotein Isolation Kit, ConA
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Glycoprotein Isolation Kit, WGA
Product LinePierce™
Quantity1 Kit
TargetGlycoprotein
TypeGlycoprotein Isolation Kit
Stationary PhaseLectin
Sufficient For10 Reactions
FormatKit, Microcentrifuge, Spin Column
Column TypeAffinity, Agarose Resin
Unit SizeEach
Contents & Storage
Sufficient For: Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640 μL (1 to 1.5 mg total protein) each
• WGA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes

Store at 4°C.

Frequently asked questions (FAQs)

What is the difference between your Pierce Glycoprotein Isolation Kit, ConA and WGA?

The ConA kit (Cat. No. 89804), which has concanavalin A immobilized on the resin, recognizes alpha-linked mannose, and to a lesser extent, terminal glucose residues. The WGA kit (Cat. No. 89805), which has wheat germ agglutinin immobilized on the resin, recognizes N-acetyl glucosamine (GlcNAc) groups and sialic acid.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?

Enrichment of specific proteins or protein complexes can most easily be accomplished by using target-specific immobilized antibody or PTM specific affinity binding. We offer several kits targeting phosphorylated proteins, alpha- linked mannose and terminal glucose residues, N-acetyl glucosamine, polyubiquinated proteins, ATP binding sites, and active serine hydrolase enzymes. Choose the right protein enrichment kit for your application using the following link (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/protein-enrichment.html).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.