Pierce™ 6 Protein Digest, equimolar, LC-MS grade
Pierce™ 6 Protein Digest, equimolar, LC-MS grade
Thermo Scientific™

Pierce™ 6 Protein Digest, equimolar, LC-MS grade

The Thermo Scientific™ Pierce™ 6 Protein Digest Standard, Equimolar, LC-MS Grade, is a highly validated equimolar protein digest mixture forRead more
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Catalog number 88342
Price (USD)
186.00
Each
Add to cart
Request bulk or custom format
Price (USD)
186.00
Each
Add to cart
The Thermo Scientific™ Pierce™ 6 Protein Digest Standard, Equimolar, LC-MS Grade, is a highly validated equimolar protein digest mixture for use as a quality control sample for mass spectrometry (MS) analysis of proteomic samples.

• Positive control sample—optimized and validated as a quality control standard for mass spectrometry applications
• Excellent sequence coverage—on average, greater than 70% sequence coverage of the 6-protein mixture
• Validated peptide quality—digestion procedure optimized for minimal missed cleavages and over-alkylation
• Rigorously tested—high-quality, consistent, 6-protein digest guaranteed and documented via lot-specific certificates of analysis
• Stable—provided in a stable lyophilized format

The Pierce 6 Protein Digest Standard contains an equimolar mixture of highly pure bovine cytochrome c, lysozyme, alcohol dehydrogenase, bovine serum albumin, apo-transferrin, and beta-galactosidase. The proteins are reduced, alkylated (iodoacetic acid), digested (MS-grade trypsin), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard free of intact protein. The peptide digest provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mass Spectrometer
Product TypeProtein Digest
Product LinePierce™
Starting MaterialPeptides
Final Product TypePeptides
Detection MethodMass Spectrometry
Workflow StepCalibration
Quantity100 pmol
FormSolid
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).

Frequently asked questions (FAQs)

What are the UniProt IDs for the proteins in the Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342) standard?

The UniProt IDs for the proteins in the Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342) standard, are:
    Serum Albumin, Bovine (Uniprot: P02769)
    Cytochrome C, Bovine (Uniprot: P62894)
    Lysozyme, Chicken (Uniprot: P00698)
     β-galactosidase, E. coli (Uniprot: P00722)
    Apo-Transferrin, Bovine (Uniprot: Q29443)
    Alcohol Dehydrogenase, S. cerevisiae (Uniprot: P00330)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center or Mass Spectrometry Support Center.

How do I verify the LC-MS system after installation of a new column for proteomic applications?

Several technical replicate injections of a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) should be used to equilibrate and compare the new column to reference chromatograms.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I assess my LC-MS system performance?

The best way to assess the performance of an entire LC-MS system is using a mass spectrometry calibration solution, peptide retention time standards (Pierce Peptide Retention Time Calibration Mixture (PRTC), Cat. No. 88321 and Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix), Cat. No. A40010), and a known standard sample, e.g., Pierce BSA Digest (Cat. No. 88341), Pierce 6 Protein Digest (Cat. No. 88342), or Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) specific to your application. We recommend frequent assessment of LC-MS system performance on a routine basis to compare results over time.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I don't need a complex digest for my MALDI study, but I need something more than a single protein digest. What do you recommend?

We recommend using the moderately complex Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Which standards and calibrants do you recommend using for assessing LC-MS system suitability?

Here are our recommendations:

- Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321) for LC system and gradient optimization
- Pierce BSA Protein Digest, MS grade (Cat. No. 88341), Pierce 6 Protein Digest, equimolar, LC-MS grade (Cat. No. 88342), and Pierce HeLa Protein Digest Standard (Cat. Nos. 88328, 88329) for "bottom up" LC-MS method optimization and LC-MS suitability assessment
- Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) (Cat. No. A40010) for assessing the gradient, sensitivity, and dynamic range of the LC-MS system for discovery and targeted peptide quantitative workflows
- Pierce TMT11plex Yeast Digest Standard (Cat. Nos. A40938, A40939) for assessing system performance for TMT-based quantitative workflows
- Pierce Intact Protein Standard Mix (Cat. Nos. A33526, A33527) for "top down" LC-MS method optimization and LC-MS suitability assessment

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.