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eBioscience™ Annexin V Apoptosis Detection Kits
Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.
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| Catalog Number | Conjugate | Excitation/Emission | Quantity |
|---|---|---|---|
| 88-8006-72 | eFluor 450, 7-AAD | 405, 546/450, 647 | 50 Tests |
| 88-8005-74 | FITC, Propidium Iodide | 488, 535/520, 617 | 200 Tests |
| 88-8006-74 | eFluor 450, 7-AAD | 405, 546/450, 647 | 200 Tests |
| 88-8007-74 | APC, Propidium Iodide | 650, 535/660, 617 | 200 Tests |
| 88-8008-72 | PCP-eFluor 710 | 50 Tests | |
| 88-8008-74 | PCP-eFluor 710 | 200 Test | |
| BMS500FI-100 | FITC, Propidium Iodide | 488, 535/520, 617 | 100 Tests |
| BMS500FI-20 | FITC, Propidium Iodide | 488, 535/520, 617 | 20 Tests |
| BMS500FI-300 | FITC, Propidium Iodide | 488, 535/520, 617 | 300 Tests |
| 88-8102-72 | PE, 7-AAD | 50 Tests | |
| 88-8102-74 | PE, 7-AAD | 200 Tests | |
| 88-8103-72 | PE/Cy7 | 50 Tests | |
| 88-8103-74 | PE/Cy7 | 200 Tests | |
| BMS500BT-100 also known as BMS500BT/100 | Biotin, Propidium Iodide | NA, 535/NA, 617 | 100 Tests |
| BMS500BT-20 also known as BMS500BT/20 | Biotin, Propidium Iodide | NA, 535/NA, 617 | 20 Tests |
| BMS500BT-300 also known as BMS500BT/300 | Biotin, Propidium Iodide | NA, 535/NA, 617 | 300 Tests |
| 88-8005-72 | FITC, Propidium Iodide | 488, 535/520, 617 | 50 Tests |
| 88-8007-72 | APC, Propidium Iodide | 650, 535/660, 617 | 50 Tests |
Catalog number 88-8006-72
Price (USD)
276.00
Each
Conjugate:
eFluor 450, 7-AAD
Excitation/Emission:
405, 546/450, 647
Quantity:
50 Tests
Price (USD)
276.00
Each
Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.
Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.
In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).
Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.
In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).
Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionAnnexin V Apoptosis Detection Kit eFluor 450
Quantity50 Tests
FormatKit
Kit Contents• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V eFluor 450, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)
Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
• Annexin V eFluor 450, 1 vial (0.25 mL)
• 7-AAD Viability Staining Solution, 1 vial (0.5 mL)
Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits.
Product TypeApoptosis Detection Kit
Excitation/Emission405, 546/450, 647
Shipping ConditionWet Ice
Product LineeBioscience
ConjugateeFluor 450, 7-AAD
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Unit SizeEach
Contents & Storage
2°C to 8°C. Avoid temperature fluctuations.