Slide-A-Lyzer™ G2 Dialysis Cassettes, 2K MWCO
Note: Product 87717 will be discontinued and replaced by product 66205 by 6/30/23.
Slide-A-Lyzer™ G2 Dialysis Cassettes, 2K MWCO
Thermo Scientific™

Slide-A-Lyzer™ G2 Dialysis Cassettes, 2K MWCO

Important Note: These G2 Dialysis Cassettes will be discontinued on June 1, 2023 and replaced by G3 Dialysis Cassettes, a new generation of cassettes. Consider switching to G3 Dialysis Cassettes. Remove salts and contaminants while maximizing sample recovery with these second-generation, self-floating protein dialysis cassettes.
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Catalog NumberSample Volume (Metric)
877170.5 mL
877183 mL
8771915 mL
8772030 mL
8772170 mL
Catalog number 87717
Price (USD)
147.00
Each
Add to cart
Sample Volume (Metric):
0.5 mL
Request bulk or custom format
Price (USD)
147.00
Each
Add to cart
Slide-A-Lyzer G2 2K molecular-weight cut off (MWCO) dialysis cassettes (2K MWCO) provide maximum convenience, flexibility, and performance for sample dialysis. The cassettes use a cellulose membrane that retains proteins and other macromolecules that are larger than 2000 Daltons, while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. The membranes and cassettes are manufactured using clean-room conditions to ensure they are contaminant-free.
Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or pipette through the unique sample port. The cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (2K MWCO):
• Easy-loading—pipette-accessible for easy sample loading and retrieval
• Self-floating—integrated air chambers eliminate the need for float buoys
• Sturdy construction—ensures the highest possible sample integrity and protection
• Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
• Multiple sizes—five cassette capacities to optimally match 0.5 to 70 mL sample volumes
• Versatile—ideal for removing low-molecular weight contaminants, performing buffer exchange, and desalting

Volume ranges:
• 0.5 mL = 0.25 to 0.75 mL
• 3 mL = 1 to 3 mL
• 15 mL = 5 to 15 mL
• 30 mL = 10 to 30 mL
• 70 mL = 25 to 70 mL

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Purification TargetBuffer Exchange, Protein
MembraneCellulose Membrane, Dialysis
FormatCassette
MWCO2.0 kDa
Product LineSlide-A-Lyzer™
Quantity10 Cassettes
Sample Volume (Metric)0.5 mL
Unit SizeEach
Contents & Storage
Store at room temperature

Frequently asked questions (FAQs)

If the Slide-A-Lyzer Dialysis Cassette is not reusable, why are there four portholes?

It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much sample will I lose in the Slide-A-Lyzer Dialysis Cassette?

The Slide-A-Lyzer Dialysis Cassette is the same regenerated cellulose as other dialysis tubing so you can expect about the same amount of protein loss as with tubing. As a guide, a 1 mg/mL solution will have a recovery rate of greater than 95%; at 100 mg/mL the recovery drops to 75-80%; and in solutions as dilute 10 µg/mL users may observe only a 50% (or less) recovery.

What conditions do you recommend for dialysis?

Dialyze for the amount of time sufficient to remove low molecular weight compounds for the specific downstream application. A typical dialysis procedure is as follows:

1) Dialyze for 2 hrs at room temperature or 4 degrees C.
2) Change the dialysis buffer and dialyze for another 2 hrs.
3) Change the dialysis buffer and dialyze overnight at 4 degrees C.

Use the dialysis buffer at 200-500 times the volume of the sample. For example, if the starting sample volume is 1 mL, then use a dialysis buffer amount that is equal to 200 mL-500 mL for each dialysis buffer change.

What is the buoy for?

The buoy plays two roles: it suspends the Slide-A-Lyzer Dialysis Cassette above the stirring bar during dialysis; it provides a stand to set the Slide-A-Lyzer Dialysis Cassette in on the bench-top

Is it necessary for me to buy needles and syringes if I already have them?

No, any 18 gauge needle at least one inch long, and syringes of sufficient volume will work.

View all Slide-A-Lyzer™ G2 Dialysis Cassettes, 2K MWCO FAQs