ExoSAP-IT™ PCR Product Cleanup Reagent
ExoSAP-IT™ PCR Product Cleanup Reagent
Applied Biosystems™

ExoSAP-IT™ PCR Product Cleanup Reagent

ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotidesRead more
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Catalog NumberDescriptionFor Use With (Application)ConcentrationQuantity
78201.1.MLExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°CPCR Purification500 rxns1 mL
78205.10.MLExoSAP-IT™ PCR Product Clean-up Reagent, 5000 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°CPCR Purification5000 rxns10 mL
78202.4X.1.MLExoSAP-IT™ PCR Product Clean-up Reagent, 2000 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°CPCR Purification2000 rxns4 x 1 mL
78200.200.ULExoSAP-IT™ PCR Product Clean-up Reagent, 100 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°CPCR Purification100 rxns200 μL
Catalog number 78201.1.ML
Price (USD)
596.00
Each
Add to cart
Description:
ExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C
For Use With (Application):
PCR Purification
Concentration:
500 rxns
Quantity:
1 mL
Price (USD)
596.00
Each
Add to cart
ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.

• Remove excess primers and unincorporated nucleotides enzymatically
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery
• Minimize column cleanup and sample loss

The ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.

Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.

After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.

Specifications
Concentration500 rxns
DescriptionExoSAP-IT™ PCR Product Clean-up Reagent, 500 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C
Sample TypePCR Products
For Use With (Application)PCR Purification
No. of Reactions500
Product LineExoSAP-IT™
Protein FormRecombinant
Quantity1 mL
Research CategoryGenetic Diseases
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store at -5 to -30°C.

Frequently asked questions (FAQs)

Can ExoSAP-IT PCR cleanup reagents be used with automatic handling systems?

The HT ExoSAP-IT Fast High-Throughput PCR Product Cleanup reagent is designed for automated handlers as it has a lower viscosity than standard ExoSAP-IT PCR Product Cleanup reagent, which contains some glycerol.

After purification with ExoSAP-IT PCR cleanup reagents, what is the recommended quantification method?

The Quant-iT PicoGreen dsDNA Assay Kit is recommended for quantifying PCR products that have been cleaned with ExoSAP-IT reagent. ExoSAP-IT PCR cleanup results in 100% recovery regardless of amplicon length, and if the PCR product was quantified prior to ExoSAP-IT cleanup, additional quantification is not necessary. We do not recommend using a spectrophotometer as degraded products will be included in the quantification.

Can ExoSAP-IT PCR cleanup reagents be used for removal of primers after first-strand cDNA synthesis?

For use with reverse transcription, ExoSAP-IT does not degrade the DNA-RNA hybrid. However, it will degrade single-stranded cDNA if a 3' -OH end is present.

Will the two tracking dyes used for visualization in the DreamTaq Hot Start Green PCR Master Mix interfere with the ExoSAP-IT PCR cleanup reagents?

The tracking dyes used in the DreamTaq Hot Start Green PCR Master Mix have not been shown to interfere with functionality of the ExoSAP-IT cleanup reagents.

Can ExoSAP-IT PCR cleanup reagents be used to cleanup PCR products amplified using High Fidelity (HiFi) DNA polymerases?

The proofreading function of HiFi DNA polymerases can cause partial degradation of PCR products during extended incubation at 80°C. ExoSAP-IT Express reagent is recommended for HiFi PCR cleanup because it only requires 1 minute at 80°C for inactivation.

View all ExoSAP-IT™ PCR Product Cleanup Reagent FAQs