Remove salts and small contaminants to maximize sample purity and recovery with Slide-A-Lyzer 2K MWCO dialysis cassettes.
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Catalog Number
Sample Volume (Metric)
66205
0.5 mL
66203
3 mL
66212
12 mL
66230
30 mL
4 Options
Catalog number 66205
Price (USD)
129.00
Each
Add to cart
Sample Volume (Metric):
0.5 mL
Request bulk or custom format
Price (USD)
129.00
Each
Add to cart
Slide-A-Lyzer Dialysis Cassettes (2K MWCO) facilitate the simple and effective removal of salts and small contaminants from proteins and other macromolecules greater than 2,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange and complete sample recovery.
Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. Slide-A-Lyzer cassettes can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers. No knots, clips, or caps are needed to seal the units and prevent leaking and sample loss during the dialysis procedure.
Features of Slide-A-Lyzer Dialysis Cassettes: • Color-coded—blue frame specifies cassettes with the 2,000-MW pore-size membrane • Four cassette sizes—select the cassette that best suits your sample volume (0.5 to 30 mL) • Fast dialysis—flat cassette chamber with two membranes provides high surface area-to-volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing • High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Membrane specifications: • Composition: regenerated cellulose synthesized by the viscose method • Thickness: 0.9 to 1.2 mil (22.5 to 30 μm) • Hydration time required before use: 2 minutes for normal volumes or 4 minutes for minimal volumes • Glycerol content: none • Sulfur content: 0.169% • Heavy metals content: trace
Volume ranges: • 0.5 mL = 0.1 to 0.5 mL • 3 mL = 0.5 to 3 mL • 12 mL = 3 to 12 mL • 30 mL = 12 to 30 mL
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDialysis Cassettes, 0.5 mL
Purification TargetBuffer Exchange, Protein
MembraneCellulose Membrane, Dialysis
FormatCassette
MWCO2.0 kDa
Product LineSlide-A-Lyzer™
Quantity10 Cassettes
Sample Volume (Metric)0.5 mL
Unit SizeEach
Contents & Storage
Store at room temperature
Frequently asked questions (FAQs)
Does the Slide-A-Lyzer Dialysis Cassette leak?
The innovative, patented design does not permit wicking that can occur with homemade microdialysis devices.
What sizes are available for the Slide-A-Lyzer Dialysis Cassette? What MWCO membranes are available?
Four sizes to accommodate samples between 0.1 and 30 ml are currently available in five different MWCOs (2K, 3.5K, 7K, 10K and 20K).
The G2 Slide A lyzers on the other hand are available in five different sizes to accommodate samples between 0.25 and 70mL.
These units, especially those that accommodate smaller volumes, provide for greater sample recovery and shorter dialysis times than one can achieve with traditional tubing. We have a cassette selection guide to help you choose the appropriate cassette for your application. There are also product demonstrations for this and other High-Performance Dialysis Products.
What prevents the Slide-A-Lyzer Dialysis Cassette from leaking?
An internal gasket, which isn't visible after the cassette is assembled, reseals itself as the needle is withdrawn. To maintain sample volume and integrity, users should remember these three key rules:
1. Before use, fill the cassette with air to verify that the system is airtight.
2. Puncture each porthole only once.
3. Do not allow the membrane to come into contact with the sharp needle bevel.
What is the purpose of the buoy for the Slide-A-Lyzer Dialysis Cassette?
The buoy suspends the Slide-A-Lyzer Cassette above the stirring bar during dialysis and it provides a benchtop stand on which to set the Slide-A-Lyzer Cassette. These are not required with the G2 Slide-A-Lyzer Dialysis Cassettes.
What is the highest temperature and the pH range that is compatible with the Slide-A-Lyzer Cassette?
Slide-A-Lyzer Cassettes can be held at 56 degrees C for two hours. At temperatures and times above these, the plastic gets soft and can deform. Thermo Scientific recommends that users maintain a pH range of 5-9.