SMART Digest™ Trypsin Kits
SMART Digest™ Trypsin Kits
Actual product may vary
Thermo Scientific™

SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

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Catalog NumberDescriptionFormatIncludes
60109-101SMART Digest Trypsin Kit, with Collection plateSMART Digest Resin Pre-filled into PCR TubesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
60109-101-LPHSmart digest kit with collection plate, low pHSMART Digest Low pH Resin Pre-filled into PCR TubesSMART Digest Low pH Resin Pre-filled into 96 PCR Tubes, Collection Plate
60109-103SMART Digest Trypsin Kit, with SOLAμ/collection plateSMART Digest Resin Pre-filled into PCR TubesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, SOLAμ SPE Plate
60109-102SMART Digest Trypsin Kit, with Filter/Collection plateSMART Digest Resin Pre-filled into PCR TubesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, Filter Plate
60109-101-BSMART Digest Trypsin Kit, Bulk Resin optionSMART Digest Resin, Bulk Non-magneticBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer
60109-101-MBSMART Digest Trypsin Kit, Magnetic Bulk Resin optionSMART Digest Resin, Bulk MagneticBulk Magnetic SMART Digest Resin, SMART Digest Buffer
60109-102-BSMART Digest Trypsin Kit, Bulk Resin option with Filter/Collection plateSMART Digest Resin, Bulk Non-magneticBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, Filter Plate
60109-102-MBSMART Digest Trypsin Kit, Magnetic Bulk Resin option with Filter/Collection plateSMART Digest Resin, Bulk MagneticBulk Magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, Filter Plate
60109-103-BSMART Digest Trypsin Kit, Bulk Resin option with SOLAμ/collection plateSMART Digest Resin, Bulk Non-magneticBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, SOLAμ SPE Plate
60113-101SMART Digest Trypsin Kit, SolubleSMART Digest Soluble TrypsinSMART Digest Soluble Trypsin, SMART Digest Buffer, Collection Plate
60109-103-MBSMART Digest Trypsin Kit Magnetic Bulk Resin option with SOLAμ/collection plateSMART Digest Resin, Bulk MagneticBulk Magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, SOLAμ SPE Plate
Catalog number 60109-101
Price (USD)
837.13
Each
Add to cart
Description:
SMART Digest Trypsin Kit, with Collection plate
Format:
SMART Digest Resin Pre-filled into PCR Tubes
Includes:
SMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
Price (USD)
837.13
Each
Add to cart

Obtain high quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

Trypsin Protein Digestion

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues.

Easy and simple
  • Significantly increased speed of sample processing
  • Simple process
  • Easily automate entire protocol for high-throughput processing
Reliable Results
  • Increased reproducibility over existing protocols
  • Higher sensitivity
  • Higher data confidence
Significantly Reduces Sample Preparation Time

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Cleaner Samples

Reduction in the number of chemicals used and the immobilized trypsin design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. This results in results which are easier to interpret.

Couple with SOLAμ SPE Plates for Desalting

When used with the SMART Digest kits for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.

Specifications
TypeProtein Digestion
DescriptionSMART Digest Trypsin Kit, with Collection plate
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymeTrypsin
CleavageC-terminal side of lysine and arginine amino acids
CaptureNone
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
Unit SizeEach

Frequently asked questions (FAQs)

How compatible is the SMART Digest Trypsin kit with detergents (e.g. CHAPS, OGS, TWEEN and RIPA)?

• CHAPS – results in ~ 30% reduction in digest efficiency.
• OGS – no reduction in digestion efficiency.
• TWEEN – no reduction in digestion efficiency.
• RIPA – results in ~ 20% reduction in digestion efficiency. The addition of RIPA, for ribonuclease A digestion, results in a concentration-dependent effect, where initial enzyme inhibition is overcome by improved substrate solubilization at higher concentrations only.

How much urea can I use with the SMART Digest Trypsin Kit?

The SMART Digest Trypsin Kit is not affected by concentrations of up to 0.5 M of urea. If the concentration is higher than this, we recommend that the sample is diluted to less than 0.5 M of urea prior to beginning digestion using the SMART Digest Trypsin Kit.

What is the composition of the SMART Digest buffer that comes with the SMART Digest Trypsin Kit?

The composition of the SMART Digest Trypsin Kit buffer is as follows:
Chemical Name (CAS No., EINECS No.): Kit Component Quantity, Weight %
• Water (7732-18-5, 231-791-2): 2, 50-95%
• Glycerol (56-81-5, 200-289-5): 2, < 20%
• Tris Base (77-86-1, 201-064-4): 2, < 10%
• Tris-HCI (1185-53-1, 214-684-5): 2, < 10%
• Calcium Chloride (10043-52-4, 233-140-8): 2, < 10%
• Sodium Azide (26628-22-8, 247-852-1): 2, < 0.1%

Why don't I need to quantitate the protein for the SMART Digest Trypsin Kit?

The SMART Digest Trypsin Kit contains an excess of enzyme capable of digesting between 200 pg and 3.5 mg of protein. As most samples will fall in this range, it is not necessary to routinely quantitate protein concentration prior to digestion.

Why don't I need to use urea to unfold the protein in the SMART Digest Trypsin Kit?

The reason urea is needed as a first step in an in-solution protocol is to disrupt the sample and partially unfold the proteins. The proteins need to be partially unfolded so that the trypsin enzyme can have better access to the internal amino acid chain, not just the surface of the protein of interest. The reason the SMART Digest Trypsin kit does not need urea is that it uses heat to unfold the protein.

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