KingFisher™ Duo Prime Purification System
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KingFisher™ Duo Prime Purification System
KingFisher™ Duo Prime Purification System
KingFisher™ Duo Prime Purification System
KingFisher™ Duo Prime Purification System
Thermo Scientific™

KingFisher™ Duo Prime Purification System

This compact purification instrument provides high quality yields of nucleic acids (DNA/RNA) and proteins with excellent reproducibility and minimal hands-on time.
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Catalog NumberDescription
5400110KingFisher Duo Prime
Catalog number 5400110
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Description:
KingFisher Duo Prime
Reap high yields of quality nucleic acids from a variety of starting materials with the Thermo Scientific™ KingFisher™ Duo Prime purification system. This compact instrument is ideal for laboratories requiring nucleic acid purification, and is easily modified for use in proteomics and cell isolation. It provides excellent reproducibility with minimal hands-on time, and has a small footprint in the lab. New features, including a UV lamp for easy decontamination and optional bar-code reader enhance functionality.

Features of the KingFisher Duo Prime system

  • Isolates DNA, RNA and proteins from a variety of starting materials including cell-free body fluids, blood, bacteria, cell cultures, tissue and plant samples; with volumes ranging up to 5 mL
  • Automated magnetic bead technology, included in all KingFisher systems, allows excellent recovery of samples with great reproducibility and efficiency
  • Built-in UV lamp delivers easy and effective decontamination
  • Optional bar-code reader allows users to track samples directly in the internal software
  • Use with optimized KingFisher Pure DNA and RNA isolation kits and protocols for a complete, easy-to-use solution, or customize with Thermo Scientific™ BindIt™ software to create your own protocols
  • Available in eight languages: English, French, Spanish, German, Chinese, Japanese, Russian and Portuguese

Operating with BindIt Software

  • Protocols can be modified or developed, as well as imported directly to the instrument using PC or via USB stick
  • Able to save run log files individually, providing traceability and complete data management
  • Flexible, fast and compact size system for diverse applications
Recommended for: DNA and RNA isolation from various starting materials, proteomic applications, cell isolation
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Volume (Metric) Processing96 DW plate 50 to 1000 μL, 24 DW plate 200 to 5000 μL, Elution strip 30 to 130 μL
DescriptionKingFisher Duo Prime
Collection Efficiency of Particles≥95%
Cooling Temperature4° to 75°C in RT (elution strip)
DisplayLCD color display
Elution StripSpecial design, 1 x 12 format
For Use With (Application)DNA and RNA isolation from various starting materials, proteomic applications, cell isolation
Heating Temperature10°C to 75°C in RT (plate row A)
Particle Size (Metric)ca. >1 μm
PortsUSB
QuantityEach
Samples per RunUp to 12 (12-pin magnet head); Up to 6 (6-pin magnet head)
Type of Magnet Head12-pin or 6-pin
UV Exposure TimeUp to 16 hr.
UV Lamp8W
MemorySpace for ca. 200 protocols
Plate TypeMicrotiter 96 Deepwell plate (95040450); KingFisher Flex 24 Deepwell plate (95040470)
Protocol ImportUsing PC or USB memory device
Depth (English)18.1 in.
Depth (Metric)46 cm
Height (English)13.4 in.
Height (Metric)34 cm
Weight (English)37.5 lb.
Weight (Metric)17 kg
Width (English)15.7 in.
Width (Metric)40 cm
Unit SizeEach

Frequently asked questions (FAQs)

What DNA extraction kit would you recommend for fragments smaller than 250 kb that are compatible with a KingFisher Flex Purification System?

We recommend the MagMAX DNA Multi-Sample Ultra 2.0 Kit (Cat. No. A36570) that is optimized for use on the moderate-throughput KingFisher Duo Prime Purification System (Cat. No. 5400110) and the higher-throughput KingFisher Flex Purification System, KingFisher with 96 Deep-well head (Cat. No. 5400630).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Where can I find the manual for the BindIt Software?

The User Manual for the BindIt Software can be downloaded from the BindIt Software product page online, under the Documents & Downloads section. It is also included within the BindIt Software installation file under "Documentation".

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Do you have data that shows how the UV lamp of the KingFisher Duo Prime instrument gets rid of bacterial contamination?

Yes we have an application note that demonstrates the lethal effect of UVC irradiation on bacterial growth and deactivation of human gDNA, "Decontamination of the KingFisher Duo Prime processing chamber using UV light".

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What type of tip heads do I receive when I purchase the KingFisher Duo Prime instrument?

You will receive the following tip heads when you purchase a KingFisher Duo Prime instrument:
• 6-tip head (for 24 deep-well plate)
• 12-tip head (for 96 deep-well plate)

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Do you sell elution strips for the KingFisher Duo Prime 24 Deep-Well (6-tip) format?

We do not sell elution strips for the KingFisher Duo Prime 24 Deep-Well format. A 24 Deep-Well plate must be used for elution with the 24 Deep-Well protocol.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.