What is a good alternative to Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, MaxiSorp, 350 µL (Cat. No. 439454)?
There is no exact alternative. The closest alternative can be found on the same product page, Cat. No. 442404. Two other potential alternatives on the same product page are Cat. No. 456537 and Cat. No. 460894. You can view the specific differences between these products on the product page by clicking into the "Full Specifications" dropdown for each.
For Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, is the surface treatment solely on the bottom of the well, or is it also applied to the sides?
For energy treated plates, the entire plate surface receives the energy treatment. The MaxiSorp, PolySorp, MediSorp and MultiSorp plates are energy treated plates. For traditionally treated plates, the well surface is treated up to the listed working volume.
What length of peptide is ideal for binding to the Thermo Scientific MaxiSorp surface? What are the detection limitations?
We have tested and found that a 3 amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH Modules and CovaLink NH2 Modules and Plates. Using covalent immobilization of small peptide residues, you can typically obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope. We have discovered that a 7 amino acid peptide from the MHC Class II antigen can be detected when adsorbed on the MaxiSorp surface. We state that the detection limitation using the MaxiSorp surface is between 3 and 7 amino acid residues.
One additional note is that detection is contingent upon the orientation of the peptide when immobilized. If the active site is inactivated or hidden at the site facing the solid phase, no detection signal is observed.
Can I bind either single- or double-stranded DNA to the Thermo Scientific MaxiSorp surface?
Single-stranded DNA can be adsorbed to our MaxiSorp surface using approximately 10 µg ssDNA per mL PBS, pH 8.2, although the stability is uncertain. Based on our experience, ssDNA immobilized on the MaxiSorp surface is so loosely bound that it can be removed by stringent washing.
Double-stranded DNA will not bind to the MaxiSorp surface. DNA, however, can be covalently bound to Nunc NucleoLink Strips.
Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?
Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.