Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?
It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system
Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".
Can I import a standard curve for my real-time PCR data into the QuantStudio Real-Time PCR Software?
If you are analyzing results from the ViiA 7 Real-Time PCR System, QuantStudio 6 Flex Real-Time PCR System, or QuantStudio 7 Flex Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Real-Time PCR Software following the instructions below:
1. Open the data file in the QuantStudio Real-Time PCR Software.
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply Analysis Settings.
Is the ViiA7 Real-Time PCR System compatible with the 7900HT Fast System Twister robot?
No, the ViiA7 Real-Time PCR System is not compatible with the 7900HT Fast System Twister robot. For automation, the ViiA7 Real-Time PCR System can be connected to one of the following robots:
• Orbitor RS2 Microplate Mover for QuantStudio Systems, Sequential Access (cat. No. A32268)
• Orbitor RS2 Microplate Mover for QuantStudio Systems, Random Access (cat. No. A32269)
• Twister Automation Robot for QuantStudio Systems (Cat. No. 4471066)
How can I get a summary of the calibrations performed on the QuantStudio 6 and 7 Flex Real-Time PCR Systems?
You can get a summary of the calibrations from the QuantStudio Software by going to Instrument > Instrument Console > Manage Instrument > Information > Print Calibration Log.