Clear Flat-Bottom Immuno Nonsterile 96-Well Plates
Clear Flat-Bottom Immuno Nonsterile 96-Well Plates
Actual product may vary
Thermo Scientific™

Clear Flat-Bottom Immuno Nonsterile 96-Well Plates

Choose from four binding surfaces of these 96-well plates for reliable and reproducible results in colormetric assays.
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Catalog NumberSurface TreatmentCertifiedVolume (Metric) WorkingBarcode
442404MaxiSorpNo350 μLNo Barcode
3355Immulon 1 BNo230 μLNo Barcode
3455Immulon 2 HBNo280 μLNo Barcode
3855Immulon 4 HBXNo280 μLNo Barcode
439454MaxiSorpYes350 μLNo Barcode
456529PolySorpNo350 μLNo Barcode
456537MaxiSorpYes350 μLNo Barcode
460984MaxiSorpYes350 μLIncludes Barcode
467320MediSorpYes350 μLNo Barcode
467340MultiSorpNo350 μLNo Barcode
475094PolySorpNo350 μLNo Barcode
9502227Universal BindingNo330 μLNo Barcode
95029330Enhanced BindingYes330 μLNo Barcode
Catalog number 442404
Price (USD)
357.00
Case of 60
Add to cart
Surface Treatment:
MaxiSorp
Certified:
No
Volume (Metric) Working:
350 μL
Barcode:
No Barcode
Price (USD)
357.00
Case of 60
Add to cart

Choose from four binding surfaces for reliable and reproducible results in colormetric assays with Thermo Scientific™ Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, which feature Thermo Scientific™ Nunc™, Immulon™ and Microtiter™ clear plates that are ideal for quantitative and qualitative solid phase immunoassays.

96-Well Solid Plates:

  • Clear polystyrene
  • Choice of four surfaces for optimal binding
  • Alphanumerically labeled rows and columns

Surfaces:

  • Thermo Scientific PolySorp, Immulon 1B, and Universal Binding (UB) are for adsorption of hydrophobic molecules.
  • Thermo Scientific Medisorp and Immulon 2HB are slightly hydrophilic and bind a diverse range of biomolecules.
  • Thermo Scientific MaxiSorp, Immulon 4 HBX, and Enhanced Binding(EB) are hydrophilic and ideal for antibody sandwich assays.
  • Thermo Scientific MultiSorp is very hydrophilic and works well for glycans and water soluble proteins.

Ordering Information:

Please note that availability may vary by country. 467320, 456537, 460984 and 439454 include certificate

WARNING: Cancer – www.P65Warnings.ca.gov
Specifications
BarcodeNo Barcode
Binding PropertyHydrophilic
CertifiedNo
FormatStandard
LidWithout Lid
Detection MethodColorimetric
Array8 x 12
Packaging60 Cases
ColorClear
Product TypeMicroplate
MaterialPolystyrene
Volume (Metric) Well400 μL
No. of Wells96
Volume (Metric) Working350 μL
SterilityNon-sterile
Surface TreatmentMaxiSorp
Well ShapeRound, Flat Bottom (F-well)
No. per Case60
No. per Pack5
Plate BlockingNone
Quantity60/Cs.
Target MoleculeAntibodies (Glycoproteins)
Well DesignF96
Unit SizeCase of 60

Frequently asked questions (FAQs)

What is a good alternative to Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, MaxiSorp, 350 µL (Cat. No. 439454)?

There is no exact alternative. The closest alternative can be found on the same product page, Cat. No. 442404. Two other potential alternatives on the same product page are Cat. No. 456537 and Cat. No. 460894. You can view the specific differences between these products on the product page by clicking into the "Full Specifications" dropdown for each.

Is there an alternative to Nunc MaxiSorp flat-bottom 96 well plates (Cat. No. 44-2404-21)?

We recommend Thermo Scientific Clear Flat-Bottom Immuno Nonsterile 96-Well Plates (Cat. No. 442404) as an alternative to the discontinued Nunc MaxiSorp flat-bottom 96 well plates.

For Clear Flat-Bottom Immuno Nonsterile 96-Well Plates, is the surface treatment solely on the bottom of the well, or is it also applied to the sides?

For energy treated plates, the entire plate surface receives the energy treatment. The MaxiSorp, PolySorp, MediSorp and MultiSorp plates are energy treated plates. For traditionally treated plates, the well surface is treated up to the listed working volume.

What length of peptide is ideal for binding to the Thermo Scientific MaxiSorp surface? What are the detection limitations?

We have tested and found that a 3 amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH Modules and CovaLink NH2 Modules and Plates. Using covalent immobilization of small peptide residues, you can typically obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope. We have discovered that a 7 amino acid peptide from the MHC Class II antigen can be detected when adsorbed on the MaxiSorp surface. We state that the detection limitation using the MaxiSorp surface is between 3 and 7 amino acid residues.
One additional note is that detection is contingent upon the orientation of the peptide when immobilized. If the active site is inactivated or hidden at the site facing the solid phase, no detection signal is observed.

Can I bind either single- or double-stranded DNA to the Thermo Scientific MaxiSorp surface?

Single-stranded DNA can be adsorbed to our MaxiSorp surface using approximately 10 µg ssDNA per mL PBS, pH 8.2, although the stability is uncertain. Based on our experience, ssDNA immobilized on the MaxiSorp surface is so loosely bound that it can be removed by stringent washing.
Double-stranded DNA will not bind to the MaxiSorp surface. DNA, however, can be covalently bound to Nunc NucleoLink Strips.

View all Clear Flat-Bottom Immuno Nonsterile 96-Well Plates FAQs