PrepSEQ™ Residual DNA Sample Preparation Kit
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PrepSEQ™ Residual DNA Sample Preparation Kit
Applied Biosystems™

PrepSEQ™ Residual DNA Sample Preparation Kit

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures ofRead more
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Catalog number 4413686
Price (USD)
1,164.00
Each
Add to cart
Price (USD)
1,164.00
Each
Add to cart

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures of proteins, buffers, and salts which are typical of samples that need to be quantitated for residual host cell line DNA. Quantitative and consistent recovery of DNA can be obtained from challenging matrices, including samples that have high protein concentration, low pH, or high salt.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologyMagnetic Bead
Sample TypeBiopharmaceutical Production Samples
ScaleMini
Final Product TypeGenomic DNA
For Use With (Application)Impurity Testing
High-throughput CompatibilityNot High-throughput Compatible (Manual), Automated Protocols
No. of Reactions100 preps
Product LinePrepSEQ™
Sample Volume100 μL
TargetGenomic DNA
Test Time2 hr.
Unit SizeEach
Contents & Storage
Includes buffers, magnetic beads and enzymes for DNA extraction for residual DNA quantitation for 100 reactions

Frequently asked questions (FAQs)

The magnetic beads, that are shipped with the PrepSeq Residual DNA Sample Preparation Kit (Cat. No. 4413686), are clumped together and are difficult to get back into suspension. How can I fix this?

The beads are known to clump due to precipitation of the salts in the solution. These are highly concentrated chaotropic salt solutions. The only way to get the salts and the beads back into the solution is by incubating them at 37 degrees C for at least 10 minutes or until the solution is homogenous. Intermittent high speed vortexing helps expedite the process. To get beads stuck to the lid back into solution, we recommend inverting the tube and using high speed vortexing in a pulsing manner.
If incubating at 37 degrees C does not fix the issue, we recommend incubating at 45 degrees C.

What amount of DNA can be recovered with the PrepSEQ Residual DNA Sample Preparation Kit (Cat. No. 4413686)?

With the above kit, up to 5 ng of DNA can be recovered per 30 µl magnetic beads used per reaction. PrepSEQ is specially designed to recover lower amounts of DNA (in ng to sub femto‐gram levels).