7900HT Fast Real-Time PCR System with Fast 96-Well Block Module
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7900HT Fast Real-Time PCR System with Fast 96-Well Block Module
Applied Biosystems™

7900HT Fast Real-Time PCR System with Fast 96-Well Block Module

The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility andRead more
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Catalog number 4351405
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The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan™ Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate.
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction.
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences.
• 96- or 384-well plate compatibility (including the TaqMan™ Low Density Array).
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security.
• Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity.
• Proven assay development guidelines save time and money

High Throughput
The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An Automation Accessory combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.

Flexibility
The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats let you select the format that’s right for your project, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan™ Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets you add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.

Powerful Software
Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. You can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, you can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Block FormatInterchangeable
Format96-well Plate
Thermal Accuracy±0.25°C of Display Temperature
Sample Ramp RateFast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
SensitivityDown to 10 Copies
Temperature Range (Metric)4 to 100°C
Reaction Volume Range10-30 μl
Passive Reference DyeROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
Dynamic RangeUp to 9 Logs of Linear Dynamic Range
Calibrated DyeSYBR™ Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™
High-throughput CompatibilityHigh-throughput Compatible, Multiplexing
For Use With (Equipment)7900HT Fast System
Thermal Uniformity±0.5°C
Reaction SpeedFast
Throughput≥5,000 Real Time PCR rxns per day (384-well block and automation accessory), ≥10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
Detection MethodSYBR, Primer-Probe Detection
Run Time33 min. (96-well in Fast Mode)
OpticsExtended-life 488 nm Argon-ion Laser Excitation Source, No Filters (CCD acts as spectrograph with continuous detection 500 to 650 nm)
Thermal Cycling SystemPeltier-based System
Dimensions72 cm (W) x 84 cm (D) x 64 cm (H) (with Drawer Open)
CapacityUp to 84 x 384-well Plates (with optional Automation Accessory and 384-well Block), Up to 36 x 96-well Plates with optional Automation Accessory
Quantity1 instrument
Weight114 kg with Automation Accessory, 82 kg without Automation Accessory
Unit SizeEach
Contents & Storage
1 Instrument with Fast 96-well Block Module

Frequently asked questions (FAQs)

What is the threshold on the Applied Biosystems real-time PCR instruments and how do I set it?

The threshold is the numerical value assigned for each run that reflects the average dRn (change in fluorescence) during the initial cycles of PCR (baseline). The threshold is set by determining a statistically significant point (or value) above the baseline. You can manually adjust the threshold should you desire, to the place in the geometric phase where your replicates are tightest. For more information on how to manually set a threshold, please refer to the tutorial entitled Data Analysis on the ABI PRISM 7700: Setting Baselines and Thresholds (P/N 4370923). While this tutorial is based on the ABI PRISM 7700 system, the concepts are still valid for the Applied Biosystems real-time PCR instruments. You can search the document on our website by using the part number above as the keyword.

If you choose not to manually adjust the baselines and thresholds, the SDS software on the Applied Biosystems real-time PCR instruments has an Auto Ct algorithm that can be used to automatically generate baseline and threshold values for individual detectors. The algorithm calculates baseline and threshold parameters for a detector based on the assumption that the data exhibits the "typical" amplification curve. Experimental error (i.e. contamination, pipetting inaccuracies) can produce amplification curves that deviate significantly from a typical amplification curve. The data from these irregularities can affect the Auto Ct algorithm by causing it to generate incorrect baseline and threshold parameters for the associated detector. Therefore, Applied Biosystems recommends that after analysis of experimental data, you review all baseline and threshold parameters determined by the Auto Ct algorithm.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Should I run a pure dye calibration each time I exchange the sample block of the Applied Biosystems 7900HT Fast Real-Time PCR System?

You have to run a calibration when a new sample block format is used in the 7900HT Fast Real-Time PCR System, but it is not necessary to run the pure dye calibration if the block has been used before. For example, if you use a 384-well sample block for the first time, then you have to run a 384-well block calibration. Later, should you switch to a 96-well block and then switch back to a 384-well block again, then there is no need to rerun the calibration of the 384-well sample block. We recommend that you run a background run each time you exchange the sample block because dust particles or other contaminants could get into the wells while the block is being stored outside of the instrument. Please consult the 7900HT User Manual, Cat. No. 4317596, for the recommended maintenance procedures.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I got an error message on the Applied Biosystems 7900HT Fast Real-Time PCR System that says to check the log file. What is this and where do I find it?

The log file tracks events during the course of an instrument run. To locate the log file, open up the hard drive of the instrument computer. Open the Applied Biosystems folder, then the SDS 2.x.x folder, then the log folder. The log file is a Microsoft Word file in .doc format and is saved as the date the run was initiated.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How do I create an indefinite hold (i.e., hold temperature at 4 degrees C forever) on the Applied Biosystems 7900HT Fast Real-Time PCR System?

To program a long-term hold on the Applied Biosystems 7900HT Fast Real-Time PCR System, launch the SDS software and open a new plate. Click on the Instrument tab. Move the cursor to the end of the thermal cycling profile, below the time box, and click. This will create a solid vertical black line. Click on “Add Hold”. Change the temperature to 4.0 and the time to the highest possible time, 98:59. Although an infinity symbol will not appear, the instrument will run for 98 hours and 59 seconds. The run must be aborted when you want to analyze the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How much heat is dissipating from the Applied Biosystems 7900HT Fast Real-Time PCR System?

While the Applied Biosystems 7900HT Fast Real-Time PCR System is idle, it puts off 6600 BTU/hour. While it is cycling, it puts off 8800 BTU/hour.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

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