TaqMan™ SNP Genotyping Assay, mouse
TaqMan™ SNP Genotyping Assay, mouse
Applied Biosystems™

TaqMan™ SNP Genotyping Assay, mouse

Green features
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNARead more
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Catalog NumberQuantity
4351384S (300 reactions), Made to Order
4351382M (1000 reactions), Made to Order
4351380L (2400 reactions), Made to Order
Catalog number 4351384
Price (USD)
384.00
Each
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Quantity:
S (300 reactions), Made to Order
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP), and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

The predesigned TaqMan SNP Genotyping Mouse Assay collection consists of over 10,000 assays. For targets of interest that are not covered by the predesigned assay collection, Custom TaqMan SNP Genotyping Assays are available to meet your research needs.

Benefits:
Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
Guaranteed—if you are not satisfied with the performance of a TaqMan Assay, we’ll replace it at no cost, or credit your account*

Approximate ship time
4–6 days in North America and 6–10 days in Europe

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
3'Primer ModificationNone
5'Primer ModificationNone
DescriptionVIC™ - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2
FormFrozen
FormatMade to Order
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler*

* If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR
system in order to detect and record fluorescent signals.
Green FeaturesSustainable packaging
Internal Probe ModificationVIC™ (5'), FAM (5'), MGB (Minor Groove Binder) (3')
Label or DyeFAM, VIC
Product LineTaqMan™
Purification MethodSolid Phase Extraction (SPE)
Purity or Quality GradeRNase-Free, DNase-Free
QuantityS (300 reactions), Made to Order
Shipping ConditionRoom Temperature
SpeciesMouse
TargetOver 10,000 mouse assays are available
For Use With (Application)Genotyping
Concentration40X
No. of Reactions300 reactions
Unit SizeEach
Contents & Storage
1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers).

Store at -15 to -25°C.

Frequently asked questions (FAQs)

What is the maximum size of insertion/deletion that can be detected by a TaqMan SNP Genotyping Assay?

Assays can detect an insertion/deletion of up to 6 bases. If you desire an assay to detect a larger insertion or deletion you may want to consider our Custom Design Service (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/custom-services-reagents-real-time-pcr-qpcr/custom-services-real-time-pcr-assays.html).

What can I do if there are no predesigned assays to my SNP?

You can use our Custom Assay Design Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays/custom-snp-assays.html?ICID=ta-lm-custom%20taqman%20snp%20assay-Single%20Tube%20Custom%20TaqMan%C2%AE%20SNP%20Genotyping%20Assays) to submit your SNP sequence for an assay design. Alternatively, you can also use Primer Express Software to design an assay.

How do I find an assay to my SNP?

You can easily find predesigned TaqMan SNP Genotyping Assays with our user-friendly search tool at https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays.html. Simply search by:

-Target (assay ID, rs number, gene symbol)
-Specific location on a chromosome

You can also watch this short video (https://www.youtube.com/watch?feature=player_embedded&v=qA4VWe8Jyrs) on how to search for assays.

Can the TaqMan SNP Genotyping Assays be used for allele quantification?

No. TaqMan SNP assays contain competitive probe sequences with a 1 bp mismatch where the SNP is located. The other probe will still bind at a lower efficiency, producing some signal for the other fluorophore. Therefore, these are not quantitative.

In my TaqMan SNP Genotyping Assay, which base is labeled with FAM or VIC dye?

The probe labels are provided in the assay documentation and also online. Simply look up the assay by typing the assay ID into the search bar on thermofisher.com. Click on the “View Details” button, and under the Product Details look for the Context Sequence. The assays are always in a [VIC/FAM dye] order. From the context sequence, you can see which base is first (thus labeled with VIC dye), and which is second (thus labeled with FAM dye).