MicroSEQ™ Full Gene 16S rDNA PCR Kit
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Applied Biosystems™

MicroSEQ™ Full Gene 16S rDNA PCR Kit

The MicroSEQ™ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 16S rDNA Full Gene IdentificationRead more
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Catalog number 4349155
Price (USD)
854.00
Each
Add to cart
Price (USD)
854.00
Each
Add to cart

The MicroSEQ™ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use the PCR kit to amplify the bacterial 16S rRNA gene, for subsequent sequencing using the MicroSEQ™ Full Gene 16S rDNA Sequencing Kit. This PCR kit does not include a protocol or a Quick Reference Card.

  • Sequence-based measurement ensures you get reproducible results in under 24 hours.
  • Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
  • Universal domain-level primers eliminate up-front optimization.
  • MicroSEQ™ ID Analysis Software ensures accurate species identification.
  • Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
  • Requires minimal training-similar work flow as the MicroSEQ™ D2 LSU fungal kit and the MicroSeq 500 16S rDNA kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution

Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
GC-Rich PCR PerformanceLow
TechniqueSBT (Sequence-Based Typing)
For Use With (Equipment)GeneAmp 9700, Veriti Thermal Cycler
LibrariesCustomizable, Bacterial Library (> 2000 species)
Product LineMicroSEQ™
Product TypeFull Gene 16S rDNA PCR Kit
Quantity1 Kit
Shipping ConditionDry Ice
SoftwareMicroSEQ™ ID Analysis Software
Target16S rRNA (full gene)
Target Organism ClassBacteria
For Use With (Application)Standard PCR
Sample TypeDNA (Genomic)
Unit SizeEach
Contents & Storage
• 2X PCR Master Mix (sufficient for 20 amplifications), 3 tubes
• Positive Control DNA, 1 tube
• Negative Control DNA (water), 1 tube
• Protocol and Quick Reference Guide

After first use, store at 2°C to 8°C in a PCR clean room.

Frequently asked questions (FAQs)

What primers are supplied with the MicroSEQ Full Gene 16S rDNA PCR Kit?

The MicroSEQ Full Gene 16S rDNA PCR Kit includes universal domain-level primers that are designed to amplify the bacterial 16S rRNA gene.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Where can I get a copy of the free trial version of the MicrobeBridge Software?

A 60-day-free trial of the MicrobeBridge Software can be downloaded here: www.thermofisher.com/order/catalog/product/A29238

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Who do I contact for support for MicrobeBridge Software and for MicrobeNet database issues?

For the MicrobeBridge Software, please contact your local Thermo Fisher Scientific Technical Support (https://www.thermofisher.com/us/en/home/technical-resources/contact-us.html) or email abtechsupport@thermofisher.com.

For MicrobeNet, please contact the CDC directly at microbenet@cdc.gov. This account is actively monitored by the CDC MicrobeNet staff.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

What are the minimum system requirements for installing the MicrobeBridge Software?

The MicrobeBridge Software has the following recommended hardware requirements: Minimum 1.8 GHz processor, 2 GB RAM, and 20 GB of free hard disk space. MicrobeBridge Software has been tested on Windows 7 32-bit SP1, Windows 7 64-bit SP1, and Windows 10 IoT.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Will I lose any data if I upgrade from the MicrobeBridge Software free trial version to the full version?

No data is lost with the MicrobeBridge Software installation of the full version on top of the free trial version.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.