Tempus™ Blood RNA Tube
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Tempus™ Blood RNA Tube
Applied Biosystems™

Tempus™ Blood RNA Tube

Tempus Blood RNA Tubes are for stabilization and isolation of total RNA from whole blood for gene expression analysis. AdvantagesRead more
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Catalog number 4342792
Price (USD)
504.00
Each
Add to cart
Price (USD)
504.00
Each
Add to cart
Tempus Blood RNA Tubes are for stabilization and isolation of total RNA from whole blood for gene expression analysis. Advantages of Tempus Blood RNA Tubes:

• Immediately lyse whole blood cells and stabilize RNA in a single step—no RBC lysis or proteinase K treatments required
• Freeze global gene expression profiles for up to five days at room temperature; greater than seven days at 4°C

How it works
Each Tempus Blood RNA Tube contains 6 mL Stabilizing Reagent. When the blood (3 mL) is drawn into the tube and mixed with this reagent, lysis occurs almost immediately. The stabilizing reagent inactivates cellular RNases and selectively precipitates RNA; genomic DNA (gDNA) while proteins remain in solution. Blood drawn into the Tempus Blood RNA Tube is stable for up to five days at room temperature, or minimally seven days at 4°C.

Accessory product
When the Tempus Blood RNA Tubes are used with the Tempus Spin RNA Isolation Kit (Cat. No. 4380204), RNAs with greater stability and higher yields of RNA are produced.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionBlood RNA Stabilization Tubes
Sample TypeWhole Blood
Quantity50 tubes
Shipping ConditionRoom Temperature
Starting Material Amount3 mL of Whole Blood
Unit SizeEach
Contents & Storage
50 Tempus Blood RNA Tubes; room temperature

Frequently asked questions (FAQs)

I am doing blood work and using the Tempus collection tubes. Is the Tempus system compatible with the RiboPure-Blood RNA Purification Kit?

No, please follow the RNA isolation methods described with the Tempus system. The collection tubes are not compatible with the RiboPure kit.

What is the recommended storage temperature and stability of samples in the Tempus Blood RNA Tube?

Our general recommendations are that once blood has been collected in the Tempus Blood RNA Tubes, you can store the tube at room temperature for up to 5 days or at 4 degrees C for up to 7 days. For long term storage, the tube can be frozen at -80 degrees C for at least 1.5 years.

Do you offer the Stabilizing Reagent that comes inside the Tempus Blood RNA Tube (Cat. No. 4342792) as a stand-alone item?

We do not offer the Stabilizing Reagent that comes inside the Tempus Blood RNA Tube as a stand-alone item.

Can animal blood stored in Tempus Blood RNA Tubes or PAXgene tubes be prepared with the MagMAX for Stabilized Blood Tubes RNA Isolation Kits (Cat. Nos. 4451893 & 4451894)?

Equine whole blood has been successfully tested using this system, getting RINs around 9. Bovine blood should work similarly since there is not much of a difference between the two species for RNA isolation from whole blood, but this has not been tested. The protocol would be the same as the user guide and samples should be mixed vigorously immediately after blood is drawn into the tube for at least 10 secs, incorporating tube inversion to be sure the sample is stabilized.

Will the RNA stabilization solution in the Tempus Blood RNA Tube (Cat. No. 4342792) lyse E. coli cells that are in the blood samples?

The RNA stabilization solution in Tempus Blood RNA Tubes will not lyse E. coli cells at ambient temperature. If lysis of gram-negative bacteria is desired, mix the sample thoroughly with the RNA stabilization solution, and heat the lysate at 95 degrees C for 5 mins.

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