Blocker™ BLOTTO in TBS
Blocker™ BLOTTO in TBS
Thermo Scientific™

Blocker™ BLOTTO in TBS

Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA,Read more
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Catalog number 37530
Price (USD)
171.00
Each
Add to cart
Request bulk or custom format
Price (USD)
171.00
Each
Add to cart
Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA, IHC, and other detection methods.

Compare and view all available blocking buffers

Features of Blocker BLOTTO Blocking Buffer:
Format—ready-to-use 1X TBS solution; can be diluted as needed
Formulation—formulated with anti-foaming agent and thimerosal-free preservative

Not recommended for avidin-based techniques because it contains some endogenous biotin.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeBlocker BLOTTO
Quantity1 L
FormLiquid
For Use With (Application)Western Blot, ELISA, Immunohistochemistry (IHC)
Product LineBlocker™
Concentration1X
BufferTBS
Blocking Agent5% non-fat milk
Unit SizeEach
Contents & Storage
Store at 4°C.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.