Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis.
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Catalog Number
Buffer
37517
PBS
37537
TBS
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Catalog number 37517
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231.00
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Buffer:
PBS
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231.00
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Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis. Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer, blotting include: • Fast—block membranes in 5 to 10 minutes • Flexible—guaranteed to be biotin-free for use with streptavidin system • Low background—the non-serum protein solution yields a high signal-to-noise ratio • Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months
Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.
For Research Use Only. Not for use in diagnostic procedures.
Upon receipt store at 4°C. Product is shipped at ambient temperature.
Frequently asked questions (FAQs)
What should I do about the colloidal carbon (black precipitate) in SuperBlock Blocking Buffer solution?
Allow any colloidal carbon, if present, to settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.
What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?
Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.