SuperBlock™ Blocking Buffer, blotting
SuperBlock™ Blocking Buffer, blotting
Thermo Scientific™

SuperBlock™ Blocking Buffer, blotting

Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis.
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Catalog NumberBuffer
37517PBS
37537TBS
Catalog number 37517
Price (USD)
231.00
Each
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Buffer:
PBS
Request bulk or custom format
Price (USD)
231.00
Each
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Thermo Scientific SuperBlock Blocking Buffer, blotting is an optimized PBS solution containing a protein that provides incredibly fast and effective blocking for western blot analysis. Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer, blotting include:
Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionSuperBlock (PBS) Blocking Buffer, blotting
Product TypeBlocking Buffer
Quantity1 L
FormLiquid
For Use With (Application)Western Blot
Product LineSuperBlock™
Concentration1X
BufferPBS
Blocking AgentSingle purified protein
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C. Product is shipped at ambient temperature.

Frequently asked questions (FAQs)

What should I do about the colloidal carbon (black precipitate) in SuperBlock Blocking Buffer solution?

Allow any colloidal carbon, if present, to settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What preservative is used in SuperBlock Blocking Buffer, and what is its source?

Kathon Preservative, a broad spectrum antimicrobial agent, is used at a concentration of 600 ppm.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is SuperBlock Blocking Buffer's shelf life?

Its shelf life is one year at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?

Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperBlock Blocking Buffer RNase-free?

No, SuperBlock Blocking Buffer is not RNase-free.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.