Dynabeads™ SILANE Viral NA Kit
Dynabeads™ SILANE Viral NA Kit
Invitrogen™

Dynabeads™ SILANE Viral NA Kit

For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following aRead more
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Catalog number 37011D
Price (USD)
656.00
Each
Add to cart
Price (USD)
656.00
Each
Add to cart
For sensitive, highly predictable and consistent extraction and isolation of viral DNA and RNA from human serum/plasma samples, following a simple separation protocol. The 1 μm Dynabeads have an optimized silica-like surface chemistry, and a high specific surface area, providing efficient kinetics and a high sensitivity. Their uniform size and surface area ensure reproducible results.

A higher sensitivity and improved performance is achieved with Dynabeads™ compared to magnetic particles from alternative suppliers. Other particles often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. Dynabeads™ hold reputable Dynal high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages:

• Isolates viral DNA and viral RNA
• High sensitivity, allowing for a low detection limit
• Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
• Can detect HBV DNA in a 200 μl serum sample containing only 40 infectious units (using 2 mg (50 μl) Dynabeads™)
• Quicker than spin-columns
• Cost-effective and performs to the same level as spin-columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)

Applications:
• Isolation of viral nucleic acids (DNA/RNA)
• From human serum/plasma samples
• Well suited for automated assays
• Separate kit available for the isolation of genomic DNA (Cat. no. 370-12D)

Additional Info:
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information, please contact us at: ivd@invitrogen.com
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologyMagnetic Bead
Sample TypePlasma, Serum
Elution Volume100 μL
Final Product TypeViral DNA and RNA
For Use With (Equipment)Automated Liquid Handling Systems
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
High-throughput CompatibilityHigh-throughput Compatible
Quantity96 preps
Shipping ConditionRoom Temperature
Size1 μm
TargetViral DNA/RNA
Test TimeUp to 45 min.
Starting Material AmountSerum: 200 μL
Plasma: 200 μL
Unit SizeEach
Contents & Storage

The kit contains: Dynabeads™ MyOne™ SILANE (5 mL, 40 mg/mL, see Cat. No. 370-02D for further characteristics), binding/washing/elution buffers and a protocol optimized for isolation of viral DNA/RNA. The kit content is sufficient for 96 isolations.

Store at: 2°C to 8°C.

Frequently asked questions (FAQs)

My Dynabeads magnetic beads are not pelleting well with the magnet. Do you have any suggestions for me?

Please review the following possibilities for why your Dynabeads magnetic beads are not pelleting:

- The solution is too viscous.
- The beads have formed aggregates because of protein-protein interaction.

Try these suggestions: - Increase separation time (leave tub on magnet for 2-5 minutes)
- Add DNase I to the lysate (~0.01 mg/mL)
- Increase the Tween 20 concentration to ~0.05% of the binding and/or washing buffer.
- Add up to 20 mM beta-merecaptoethanol to the binding and/or wash buffers.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

What is the principle behind isolation of viral nucleic acids with the Dynabeads SILANE Viral NA Kit?

The Dynabeads SILANE Viral NA Kit will bind genomic DNA as well as viral nucleic acids, but the buffers have been optimized for stronger binding of shorter fragments and very small amounts of viral DNA/RNA. This buffer is not advised for genomic DNA binding, because the interaction with the bead surface will be stronger, which will require more work to elute genomic DNA than viral nucleic acids.

Can Dynabeads SILANE beads be used for nucleosome isolation?

That depends. If you want to isolate intact nucleosomes (histones + DNA complex), that is not possible with Dynabeads SILANE beads. But you can isolate DNA from nucleosomes if they are not cross-linked. For chromatin immunoprecipitation, you could consider the MAGnify Chromatin Immunoprecipitation System.

How much DNA is recovered per isolation using the Dynabeads DNA DIRECT Universal Kit?

The yield will depend on the number of nucleated cells present in the sample. One unit (200 µL) of Dynabeads DNA DIRECT Universal Kit will isolate at least 200 ng of high quality, PCR-ready genomic DNA per sample, sufficient for 10 PCR reactions. The size of the sample used should be adjusted to match the Dynabeads magnetic beads capacity. A typical starting sample is 10,000 cultured cells or 10 µL blood. The protocol can be adjusted to support 30 µL blood samples, sufficient for 30-50 downstream PCR amplifications (600 ng to 1 µg DNA).

I have a long double-stranded DNA fragment I would like to isolate. What product do you recommend?

For biotin-labeled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin (Cat. No. 65305) and MyOne C1 magnetic beads (Cat. No. 65001). We recommend our Dynabeads KilobaseBINDER Kit (Cat. No. 60101), which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

View all Dynabeads™ SILANE Viral NA Kit FAQs