SuperSignal™ West Pico PLUS Chemiluminescent Substrate
SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogramRead more
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Catalog NumberKit ContentsQuantity
34578SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide1 L
34579SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide20 mL
34577SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide200 mL
34580SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide500 mL
34580X4SuperSignal West Pico PLUS Luminol/Enhancer4 x 500 mL
34578X4SuperSignal West Pico PLUS Luminol/Enhancer4 x 1 L
Catalog number 34578
Price (USD)
574.00
Each
Add to cart
Kit Contents:
SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Quantity:
1 L
Request bulk or custom format
Price (USD)
574.00
Each
Add to cart

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

  • Sensitivity: pictogram to femtogram sensitivity
  • Stability: 8-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 6 to 24 hours
  • Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates

View all HRP substrates available

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeSubstrate
Detection MethodChemiluminescence
FormLiquid
For Use With (Application)Western Blot
Membrane CompatibilityNitrocellulose, PVDF
Product LineSuperSignal™
Quantity1 L
ConjugateHRP
SubstrateHorseradish Peroxidase
Recommended Antibody Concentrations2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
SensitivityPicogram to high femtogram-level
Signal Duration6 to 24 hr.
Sufficient For120 Mini-Gel Blots, 10000 cm2 of Membrane
Kit ContentsSuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Sufficient for 120 mini-gel blots; 10,000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 500 mL
• SuperSignal West Pico PLUS Stable Peroxide, 500 mL

Store at room temperature.

Frequently asked questions (FAQs)

What types of membrane work best with SuperSignal West Pico PLUS Chemiluminescent Substrate?

Most researchers use nitrocellulose or polyvinyldiflouride (PVDF) membranes with SuperSignal West Dura Chemiluminescent Substrate. Both work well, although nitrocellulose seems to be better suited in some applications than PVDF. In addition, charge-modified nylon membrane performs well with this substrate. Please also see our guide for choosing western blot membranes (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting/membranes-western-blotting.html).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Will SuperSignal West Pico PLUS Chemiluminescent Substrate work with nucleic acid blots (Southern blotting, etc.)?

Yes. However, SuperSignal West Pico PLUS Chemiluminescent Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used the SuperSignal West Pico PLUS Chemiluminescent Substrate. Why is the entire film black or showing a reversed image?

The antibody concentration (primary and secondary) is too high. Use the antibody dilutions described in the product instructions. Most background will disappear when the proper blocking reagent and a HRP conjugate concentration are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do I see more bands with the SuperSignal West Pico PLUS Chemiluminescent Substrate than I've seen in the past with other substrates?

Because this substrate is more sensitive than other chemiluminescent substrates you might detect low-abundance proteins that were not visible before. When using a more sensitive substrate, more careful optimization of blocking buffer steps and antibody concentrations is required.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What protocol should I use to strip and reprobe a blot detected with SuperSignal West Pico PLUS Chemiluminescent Substrate ?

See Tech Tip # 23: Strip and reprobe Western blots.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all SuperSignal™ West Pico PLUS Chemiluminescent Substrate FAQs