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Pierce™ Silver Stain Kit
The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels,Read more
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| Catalog Number | Quantity |
|---|---|
| 24612 | 1 L |
| 24612X3 | 3 x 1 L |
Catalog number 24612
Price (USD)
212.00
Each
Quantity:
1 L
Price (USD)
212.00
Each
The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.
Compare all silver stains ›
Features:
• Sensitivity—detect proteins at 0.25 ng
• Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
• Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
• Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery
Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.
Compare all silver stains ›
Features:
• Sensitivity—detect proteins at 0.25 ng
• Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
• Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
• Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery
Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeSilver
Product TypeStain Kit
Product LinePierce™
Quantity1 L
Target MoleculeProtein
Unit SizeEach
Contents & Storage
Sufficient For: 20 mini gels (SDS-PAGE)
• Silver Stain Sensitizer, 2 mL
• Silver Stain, 500 mL
• Silver Stain Enhancer, 25 mL
• Silver Stain Developer, 500 mL
Store at room temperature.
• Silver Stain Sensitizer, 2 mL
• Silver Stain, 500 mL
• Silver Stain Enhancer, 25 mL
• Silver Stain Developer, 500 mL
Store at room temperature.