Glycoprotein Standards Set for Pierce™ Glycoprotein Carbohydrate Estimation Kit
Glycoprotein Standards Set for Pierce™ Glycoprotein Carbohydrate Estimation Kit
Thermo Scientific™

Glycoprotein Standards Set for Pierce™ Glycoprotein Carbohydrate Estimation Kit

The Thermo Scientific Pierce Glycoprotein Standards Set includes six purified standards (two negative and four positive controls) intended for useRead more
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Catalog number 23259
Price (USD)
340.00
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Price (USD)
340.00
Each
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The Thermo Scientific Pierce Glycoprotein Standards Set includes six purified standards (two negative and four positive controls) intended for use with any glycoprotein carbohydrate determination protocol.

Related Products
Pierce™ Glycoprotein Carbohydrate Estimation Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Glycoprotein Standards Set
AssayGlycoprotein Standards
Detection MethodColorimetric
Product LinePierce™
Product TypeGlycoprotein Carbohydrate Estimation
SpecificityNot Target-Specific
Sufficient For60 Tube Assays or 250 Microplate Assays
For Use With (Application)Internal Standard
For Use With (Equipment)Spectrophotometer, Microplate Reader
Quantity5 Protein
Unit SizeEach
Contents & Storage
Sufficient For: Preparing standard curves for glycoprotein carbohydrate estimation
• Lysozyme, lyophilized, 2.5 mg
• BSA, lyophilized, 2.5 mg
• Ovalbumin, lyophilized, 2.5 mg
• Apo-tranferrin, lyophilized, 2.5 mg
• Fetuin, lyophilized, 0.25 mg
• alpha-Acid Glycoprotein, 0.25 mg

Frequently asked questions (FAQs)

Will the reducing agent in my sample interfere with the Glycoprotein Carbohydrate Estimation assay?

Yes. Reducing agents interfere with this assay. Please see the list of compatibility of other commonly used reagents below: Compatible: EDTA, Urea (<1.0M), Guanidine HCl (<1.0M), DMSO (<10%), PBS Not Compatible: Reducing Agents, Tris, Glycerol

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Will the detergent in my sample interfere with the Glycoprotein Carbohydrate Estimation assay?

Most commonly used detergents do not interfere; however, detergents with sugar derivatives, such as octyl-b-glusoside (OG) and octyl-b-thioglucopyranoside (OTG), can contribute to the signal, resulting in an erroneously high percent carbohydrate value.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between Glycoprotein Carbohydrate Estimation Kit and the Thermo Scientific Glycoprotein Staining Kit (#24562)?

Glycoprotein Carbohydrate Estimation Kit estimates the carbohydrate content of glycoproteins in solution; however, the Thermo Scientific Glycoprotein Stain directly stains oxidized glycoprotein on gels and nitrocellulose membranes and does not estimate carbohydrate content.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use this kit to estimate the carbohydrate content of cell lysates or do I need to purify the protein?

The kit is designed for purified proteins but can also estimate the w/w percentage of carbohydrate in total protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the Glycoprotein Carbohydrate Estimation Kit to estimate free sugars?

Yes. Free sugars form aldehydes when oxidized, which react with the Glycoprotein Detection Reagent.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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