EGS (ethylene glycol bis(succinimidyl succinate))
EGS (ethylene glycol bis(succinimidyl succinate))
Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can beRead more
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Catalog number 21565
Price (USD)
250.00
Each
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Price (USD)
250.00
Each
Add to cart
Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of the EGS crosslinker:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EGS (ethylene glycol bis(succinimidyl succinate))
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine-Amine
CleavableBy Hydroxylamine
DescriptionEGS
Molecular Weight (g/mol)456.36
PEGylatedNo
Spacer Arm Length16.1 Å
Cell PermeabilityYes
Shipping ConditionAmbient
Product LinePierce™
Labeling MethodChemical Labeling
Crosslinker TypeHomobifunctional
Reactive MoietyNHS Ester
SpacerMedium (10 to 30 Å)
FormPowder
Quantity1 g
SolubilityDMF, DMSO
FormatStandard
Water SolubleNo
Product TypeCrosslinker
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for EGS (ethylene glycol bis(succinimidyl succinate))?

EGS (ethylene glycol bis(succinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.