Lipofectin™ Transfection Reagent
Product Image
Invitrogen™

Lipofectin™ Transfection Reagent

Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable forRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberIncludesQuantity
18292011One vial (1mL) of Lipofectin Transfection Reagent1 mL
18292037Four vials (1mL each) of Lipofectin Transfection Reagent4 mL
Catalog number 18292011
Price (USD)
670.00
Each
Add to cart
Includes:
One vial (1mL) of Lipofectin Transfection Reagent
Quantity:
1 mL
Price (USD)
670.00
Each
Add to cart
Lipofectin® Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin® Transfection Reagent is also suitable for transfecting DNA, RNA, and oligonucleotides into mammalian cells, and DNA into plant protoplasts. Lipofectin® reagent has also been shown to work well, in combination with PLUS® Reagent, for the transfection of HeLa cells. Lipofectin® Transfection Reagent is a 1:1 (w/w) liposome formulation of the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phophotidylethanolamine (DOPE) in membrane-filtered water.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEstablished Cell Lines, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
IncludesOne vial (1mL) of Lipofectin Transfection Reagent
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product LineLipofectin™
Product TypeTransfection Reagent
Quantity1 mL
Serum CompatibleNo
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
Contains one vial (1 ml) of Lipofectin™ Transfection Reagent. Store at 4°C. Do not freeze.

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of endothelial cells?

We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Lipofectin™ Transfection Reagent FAQs