BioNick™ DNA Labeling System
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Invitrogen™

BioNick™ DNA Labeling System

The BioNick™ DNA Labeling System is ideal for generating biotinylated DNA probes by nick translation optimized for use in non-radioactiveRead more
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Catalog number 18247015
Price (USD)
544.00
Each
Add to cart
Price (USD)
544.00
Each
Add to cart
The BioNick™ DNA Labeling System is ideal for generating biotinylated DNA probes by nick translation optimized for use in non-radioactive in situ hybridizations. These probes can also be used in Southern or northern blots, plaque lifts, colony hybridizations, and dot blot hybridizations. Using the BioNick™ DNA Labeling System:

• DNA is labeled with biotin-14-dATP, producing probe sizes from 50 to 500 bp
• One reaction labels 1 μg of template DNA
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodBiotin-based
Label or DyeBiotin
Final Product TypeProbes (Labeled DNA)
Labeling TargetDNA (General)
Includes Label or DyeYes
Labeling MethodDirect Labeling
Product LineBioNick™
Product TypeDNA Labeling System
Quantity50 Reactions
Shipping ConditionApproved for shipment on Wet or Dry Ice
Unit SizeEach
Contents & Storage
The BioNick™ DNA Labeling System includes 10X dNTP mix containing biotin-14-dATP, 10X enzyme mix containing DNA Polymerase I and DNase I, control DNA, stop buffer, and distilled water. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

What is the difference between the BioNick system and the BioPrime system?

The BioNick Labeling System generates biotinylated DNA probes by nick translation. These probes range from 50 to 500 bases. One reaction labels 1 µg of DNA. The BioPrime Labeling System generates 50-500 ng of biotinylated DNA from 25 ng template DNA by random priming. The probe size ranges from 50 to 700 bases, with a significant portion of the probe being less than 200 bases. See Mackey, J., Rashtchian, A. (1992) FOCUS 14, p.21.