UltraPure™ Salmon Sperm DNA Solution
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UltraPure™ Salmon Sperm DNA Solution
Invitrogen™

UltraPure™ Salmon Sperm DNA Solution

UltraPure™ Salmon Sperm DNA Solution is a ready-to-use, sheared DNA solution that is used directly in the preparation of prehybridizationRead more
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Catalog number 15632011
Price (USD)
223.00
Each
Add to cart
Request bulk or custom format
Price (USD)
223.00
Each
Add to cart
UltraPure™ Salmon Sperm DNA Solution is a ready-to-use, sheared DNA solution that is used directly in the preparation of prehybridization and hybridization solutions. This DNA solution is prepared from highly pure, phenol/chloroform-extracted DNA and DNase-free, RNase-free (DEPC-treated), distilled, deionized water. The DNA is sheared to an average size of ≤2,000 bp. The concentration is adjusted to 10 mg/mL.

Applications
Such solutions are used to block the nonspecific attachment of probe to the surface of a membrane.

Performance and quality testing
Concentration, size verification, viscosity, and ultraviolet absorbance (A260/A280) are analytically tested.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Chromatin Biology, Nucleic Acid Gel Electrophoresis, Blotting
Key FunctionsBlock Non-Specific Hybridization
Product LineUltraPure™
Product TypeSalmon Sperm DNA Solution
Quantity5 x 1 mL
Reagent TypeSalmon Sperm DNA
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
5 tubes Salmon Sperm DNA solution (1 ml each, 10 mg/ml)

UltraPure™ Salmon Sperm DNA is stable for 1 year at -20°C.

Frequently asked questions (FAQs)

What species of salmon is Ultrapure Salmon Sperm DNA derived from?

The species of salmon is Oncerhyncus keta.

Is Ultrapure Salmon Sperm DNA double stranded? How can I make it single stranded?

Ultrapure Salmon Sperm DNA is double stranded. It can be made single stranded by heating to 95 degrees C and then cooling rapidly on ice. Cooling is usually done with the DNA diluted in a hybridization solution which keeps it single stranded because of the large dilution volume.

What concentration should the UltraPure Salmon Sperm DNA Solution (Cat. No. 15632011) be used at?

Carrier DNA is typically used at a concentration of 100 µg/mL in both the prehybridization and hybridization solutions.