UltraPure™ TBE Buffer, 10X
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UltraPure™ TBE Buffer, 10X
Invitrogen™

UltraPure™ TBE Buffer, 10X

UltraPure™ 10X TBE Buffer is a sterile-filtered solution of 1 M Tris, 0.9 M boric acid, and 0.01 M EDTARead more
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Catalog NumberQuantity
1558102810 L
155810441 L
Catalog number 15581028
Price (USD)
392.00
Each
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Quantity:
10 L
Request bulk or custom format
Price (USD)
392.00
Each
Add to cart
UltraPure™ 10X TBE Buffer is a sterile-filtered solution of 1 M Tris, 0.9 M boric acid, and 0.01 M EDTA used to prepare 1X buffer for polyacrylamide and agarose gel electrophoresis. UltraPure™ 10X TBE Buffer is available in a 1 L plastic bottle or in a 10 L Cubitainer™ box.

Performance and quality testing
No DNase, RNase, or protease activity detected.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferRunning Buffer
Concentration10 X
FormatBottle
Gel CompatibilityPolyacrylamide Gels, Agarose Gels
For Use With (Application)Nucleic Acid Gel Electrophoresis, Blotting
Product LineUltraPure™
Product TypeTBE Buffer
Quantity10 L
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

Can I autoclave agarose to prepare agarose gels?

No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.

Do you have any tips for preparing high-percentage (3% to 4%) agarose gels?

Add the powder to cold buffer while stirring. Let the agarose rehydrate for 1 to 2 hr and then heat slowly until agarose is completely dissolved. Using a microwave at low power settings is acceptable.

What can I do to prevent static on agarose bottles?

One recommendation: get a supply of fabric softener sheets and wipe the bottle with a sheet before opening it.

How can I avoid the build-up of the precipitous film sometimes seen in 10X TBE?

The precipitation of concentrated TBE stocks may be due to nucleation of salt crystals by dust particles or other insoluble materials. Therefore, filtering the solution through a 0.2 µm cellulose acetate or cellulose nitrate filter after preparation helps avoid this precipitate. [Mayeda A, Krainer A (1991) BioTechniques 10.2, 1820]

How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.