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Trypan Blue Solution, 0.4%
Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test.Read more
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Catalog number 15250061
Price (USD)
25.49
Each
Add to cart
Price (USD)
25.49
Each
Add to cart
Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. The dye exclusion test is based upon the concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye.
cGMP manufacturing and quality system Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Cell TypeMammalian Cells
Concentration0.004
Detection MethodColorimetric
FormLiquid
Product TypeTrypan Blue
Shelf Life36 Months
SterilitySterile-filtered
For Use With (Application)Cell Counting
Green FeaturesSustainable packaging
Quantity100 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 15 – 30°C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Frequently asked questions (FAQs)
How do I quench any fluorescence outside of my live cells and leave my intracellular staining intact?
There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.
How long can cells be exposed to trypan blue before all cells are stained with the dye?
Unfortunately, there is no definitive answer to this question. The rate at which the cell-impermeant dye is absorbed depends on the cell type, their state of health, nourishment, engulfment activity, etc.
Exposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.