Trypsin (2.5%), no phenol red
Trypsin (2.5%), no phenol red
Gibco™

Trypsin (2.5%), no phenol red

Gibco Trypsin is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestiveRead more
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Catalog number 15090046
Price (USD)
47.93
Each
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Price (USD)
47.93
Each
Add to cart
Gibco Trypsin is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE reagent that feature:

• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing

The complete formulation is available.

Quality Testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco Trypsin (2.5%), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
ChelatorsNo EDTA
Cell TypeMammalian Cells
ClassificationAnimal Origin
Concentration10 X
FormLiquid
Product TypeCell Culture Dissociation Reagent
Shelf Life24 Months
SterilitySterile-filtered
Without AdditivesNo Phenol Red
Green FeaturesSustainable packaging
Osmolality270 - 320 mOsm/kg
pH7.1 to 8
Product LineGibco™
Quantity100 mL
Shipping ConditionWet Ice
SourceAnimal Origin
Tested ForIn vitro Bioassay
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you have a non-animal-origin enzyme for cell dissociation?

Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

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