HBSS (10X), no calcium, no magnesium, no phenol red
HBSS (10X), no calcium, no magnesium, no phenol red
Gibco™

HBSS (10X), no calcium, no magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
Have Questions?
Catalog number 14185052
Price (USD)
65.50
Each
Add to cart
Customize this product
Price (USD)
65.50
Each
Add to cart

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.


This HBSS is modified as follows:
WithWithout
• Glucose• Calcium
 • Magnesium
 • Phenol Red
 • Sodium Bicarbonate


The complete formulation is available.

Concentrated forms of Gibco™ HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
Dilution10 X
Product LineGibco™
Product TypeHanks' Balanced Salt Solution (HBSS)
Quantity500 mL
Solution TypeHank's Balanced Salt
For Use With (Application)Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting; and Preparing Reagents
Green FeaturesSustainable packaging
Osmolality2700 - 3000 mOsm/kg
pH5.8 - 6.1
Phenol Red IndicatorNo Phenol Red
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Unit SizeEach
Contents & Storage
Storage conditions: 15-30° C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

Do you offer a 10X version of the HBSS, no calcium, no magnesium, no phenol red (Cat. No. 14175095)?

Yes, we do offer HBSS (10X), no calcium, no magnesium, no phenol red (Cat. No. 14185052).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.