HBSS, calcium, magnesium, no phenol red
HBSS, calcium, magnesium, no phenol red
Gibco™

HBSS, calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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Catalog NumberQuantity
140251266 x 1000 mL
14025092500 mL
1402513410 x 500 mL
140250761000 mL
Catalog number 14025126
Price (USD)
262.00
Each
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Quantity:
6 x 1000 mL
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Price (USD)
262.00
Each
Add to cart

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.


This HBSS is modified as follows:
WithWithout
• Calcium• Phenol Red
• Magnesium• Sodium Pyruvate
• Glucose 


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
Dilution1 X
Product LineGibco™
Product TypeHanks' Balanced Salt Solution (HBSS)
Quantity6 x 1000 mL
Solution TypeHank's Balanced Salt
For Use With (Application)Mammalian Cell Culture
Green FeaturesSustainable packaging
Osmolality270 - 305 mOsm/kg
pH6.7 - 7.8
Phenol Red IndicatorNo Phenol Red
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Frequently asked questions (FAQs)

What is the pH of HBSS, calcium, magnesium, no phenol red at room temperature.

At room temperature, HBSS, calcium, magnesium, no phenol red has a pH of 6.7 - 7.8.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add additional HEPES to Hank's Balanced Salt Solution (HBSS) (Cat. No. 14025xxx)?

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why do you offer HBSS with and without phenol red?

Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use HBSS (with calcium and magnesium) to wash my cells before dissociation?

No, a saline solution that contains calcium and magnesium should not be used to wash cells before dissociation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all HBSS, calcium, magnesium, no phenol red FAQs