Nunc™ TripleFlask™ Treated Cell Culture Flasks

The design of the Nunc TripleFlask does not allow for mechanical scraping. However, cells are easily harvested using protein digesting enzymes, such as trypsin, collagenase, and Pronase or by using non-enzymatic dissociation solutions.
The following is a document link regarding culturing techniques for Nunc TripleFlask:
https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/culturing-technique-nunc-tripleflask-technical-note.pdf

This depends largely on the cell line and working conditions (considerations include headspace for gas exchange, supplements used, and optimal feeding schedule, among others) but we do offer guidelines for development purposes (0.3 to 0.5 mL per cm2) . Note that these volumes are approximate and may have to be modified for best performance. T25 Flask = 7 mL, T75 Flask = 25 mL, T175 Flask = 55 mL, T225 Flask = 70 mL, 300cm2 Flask = 100 mL, 500 cm2 TripleFlask = 200 mL, 35 mm round culture dish = 3 mL, 60 mm round culture dish = 5 mL, 100 mm round culture dish = 12 mL, 150 mm round culture dish = 35 mL, 4 (rectangular) well Multidish = 5 mL, 8 (rectangular) well Multidish = 3 mL, 4 (round) well Multidish = 1 mL, 6 well Multidish = 3 mL, 12 well Multidish = 2 mL, 24 well Multidish = 1 mL, 48 well Multidish = 0.5 mL.