MesenPRO RS™ Medium
Taking your work to the clinic? Check out CTS StemPro MSC SFM »
MesenPRO RS™ Medium
Gibco™

MesenPRO RS™ Medium

MesenPRO RS™ Medium is a reduced serum (2%) medium specifically formulated to support the growth of human mesenchymal stem cellsRead more
Have Questions?
Catalog number 12746012
Price (USD)
281.00
Each
Add to cart
Price (USD)
281.00
Each
Add to cart
MesenPRO RS™ Medium is a reduced serum (2%) medium specifically formulated to support the growth of human mesenchymal stem cells (MSCs) in culture. MesenPRO RS™ Medium consistently improves MSC expansion compared with conventional serum-supplemented media. Using MesenPRO RS™ Medium, MSCs can be expanded for multiple passages while maintaining their multipotential phenotype (i.e., differentiation into osteogenic, chondrogenic, and adipogenic lineages).

MesenPRO RS™ Medium consists of:

• Basal liquid medium (MesenPRO RS™ Basal Medium)
• Frozen supplement (MesenPRO RS™ Growth Supplement), which is added at the time of use.

Applications:
Formulated for the growth and expansion of mesenchymal stem cells.
Note: MesenPRO RS™ Medium has been developed for culturing MSCs at greater than clonal densities.

Recommended storage conditions:
• MesenPRO RS™ Basal Medium: Store in the dark at 2 to 8°C
• MesenPRO RS™ Growth Supplement: Store in the dark at -5 to -20°C

Both the Basal Medium and Growth Supplement are guaranteed stable for 24 months when properly stored.

Note: MesenPRO RS™ complete medium (basal, supplement and glutamine) is stable for 15 days when stored in the dark at 2 to 8°C. We do not recommend using the complete medium beyond 15 days.

Specifications
Cell TypeMesenchymal Stem Cells
ClassificationSerum-supplemented
Culture TypeAdherent Cell Culture
FormLiquid
Product TypeStem Cell Media
Serum LevelReduced Serum
Without AdditivesNo Glutamine, No Phenol Red
Green FeaturesSustainable packaging
Product LineMesenPRO™
Quantity1 kit
SpeciesHuman
Unit SizeEach
Contents & Storage
MesenPRO RS™ Medium consists of:
• 500 ml MesenPRO RS™ Basal Medium (store in the dark at 2°C to 8°C)
• 10 ml MesenPRO RS™ Growth Supplement (store in the dark at -5°C to -20°C)

The Basal Medium ships at room temperature, the Growth Supplement on dry ice.

Frequently asked questions (FAQs)

Can I use MesenPRO RS Medium with both StemPro Human Adipose-Derived Stem Cells and StemPro Bone Marrow (BM) Mesenchymal Stem Cells?

Yes, the MesenPRO RS Medium (with growth supplement added) can be used for both cell types.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).