B-27™ Supplement (50X), minus vitamin A
B-27™ Supplement (50X), minus vitamin A
Gibco™

B-27™ Supplement (50X), minus vitamin A

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Vitamin A is a customized version ofRead more
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Catalog NumberQuantity
12587001100 mL
1258701010 mL
Catalog number 12587001
Price (USD)
1,666.00
Each
Add to cart
Quantity:
100 mL
Price (USD)
1,666.00
Each
Add to cart
A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Vitamin A is a customized version of the original B-27 Supplement without vitamin A (retinyl acetate). B-27 Supplement Minus Vitamin A is a serum-free supplement ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. B-27 Supplement Minus Vitamin A is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Progenitor Cells
ClassificationSerum-free
Concentration50 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeCell Culture Supplement
Serum LevelSerum-Free
Shelf Life12 Months
SterilitySterile
Green FeaturesFewer resources used, Less waste
Product LineB-27™
Quantity100 mL
Shipping ConditionDry Ice
SourceSerum-Free
Tested ForEndotoxin, Performance, Sterility
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.

Frequently asked questions (FAQs)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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