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Gibco™
DMEM, high glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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| Catalog Number | Quantity |
|---|---|
| 11965118 | 10 x 500 mL |
| 11965092 | 500 mL |
| 11965084 | 1000 mL |
| 11965126 | 6 x 1000 mL |
| 11965167 | 5 L |
| 11965175 | 10 L |
Catalog number 11965118
Price (USD)
298.00
Each
Quantity:
10 x 500 mL
Price (USD)
298.00
Each
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
For Use With (Application)Mammalian Cell Culture
Glucose Concentration4500 mg/L
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 x 500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture