Streptomycin Sulfate
Streptomycin Sulfate
Gibco™

Streptomycin Sulfate

Streptomycin Sulfate is a water-soluble antibiotic originally purified from Streptomyces griseus. Streptomycin Sulfate acts by binding to the 30S subunitRead more
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Catalog number 11860038
Price (USD)
108.00
Each
Add to cart
Price (USD)
108.00
Each
Add to cart
Streptomycin Sulfate is a water-soluble antibiotic originally purified from Streptomyces griseus. Streptomycin Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Streptomycin Sulfate is highly active against gram-negative with some activity against gram-positive bacteria. Gibco™ Streptomycin Sulfate is used alone or in combination with penicillin, an antibiotic highly active against gram-positive bacteria, for the prevention of bacterial contamination of cell cultures. The recommended working concentration ranges from 50 to 100 μg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
For supply chain continuity, we manufacture Gibco™ Streptomycin Sulfate at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration50 to 100 μg/mL
Culture TypeMammalian Cell Culture, Insect Cell Culture
FormPowder
Product TypeAntibiotic
Shelf Life24 Months
For Use With (Application)Bacterial Selection
Quantity100 g
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2 to 8°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.