SuperScript™ IV VILO™ Master Mix
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SuperScript™ IV VILO™ Master Mix
Invitrogen™

SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
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Catalog NumberIncludesNo. of Reactions
11766500Kit with ezDnase500 Reactions
11756050Kit only50 Reactions
11756500Kit only500 Reactions
11766050Kit with ezDnase50 Reactions
Catalog number 11766500
Price (USD)
5,990.00
Each
Add to cart
Includes:
Kit with ezDnase
No. of Reactions:
500 Reactions
Request bulk or custom format
Price (USD)
5,990.00
Each
Add to cart
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step.

SuperScript IV VILO Master Mix features include:
• Fast RT reactions in just 10 minutes
• Convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• High yields reduce Ct values by more than two cycles ahead of all other reverse transcription reagents
• Efficient reaction even with low template amounts and suboptimal purity samples
• gDNA removal in as few as two minutes (SSIV VILO Master Mix with ezDNase only)

SSIV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

SuperScript IV VILO Master Mix with ezDNase is available for better removal of genomic DNA and to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix results in more cDNA in less time with less pipetting, less variation, less reaction inhibition, less gDNA interference, and with less sample than other cDNA synthesis kits or master mixes for RT-qPCR. Additional ezDNase may also be purchased separately.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodqPCR
FormatMaster Mix
GC-Rich PCR PerformanceHigh
Reaction Speed10 min.
TechniqueReverse Transcription
Optimal Reaction Temperature50°C
Reverse TranscriptaseSuperScript IV
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
For Use With (Application)Real Time PCR (qPCR)
Concentration5X
Final Product TypeFirst-Strand cDNA
No. of Reactions500 Reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Size (Final Product)Up to 10 kb
Starting MaterialRNA
IncludesKit with ezDnase
Unit SizeEach
Contents & Storage

• SuperScript IV VILO Master Mix (2 x 1 mL)
• SuperScript IV VILO Master Mix 'No RT' Control (1 mL)
• ezDNase Enzyme (500 μL)
• 10X ezDNase Buffer (1 mL)
• Nuclease-free water (1.25 mL)

Store at -15°C to -25°C.

Frequently asked questions (FAQs)

Does the TaqMan Cells-to-CT Express Kit contain reagents for the removal of genomic DNA (gDNA)?

Yes, the TaqMan Cells-to-CT Express Kit includes Express ezDNase which can be added to the Express Lysis Solution for removal of gDNA during cell lysis. Express ezDNase is a double-strand specific, heat-labile DNase that will only digest gDNA, making it compatible with the downstream reverse transcription reaction. The enzyme is automatically inactivated during a heat kill step included in the reverse transcription program. To avoid the detection of gDNA, we recommend using a TaqMan Gene Expression Assay specifically designed to span an intron-exon boundary. Such assays are designated with an "_m1" suffix. More information can be found here.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

In your SuperScript IV RT protocols, there is no ezDNase inactivation step. Will active ezDNase affect RNA or the RT reaction?

The Invitrogen ezDNase Enzyme is a novel DNase that is highly specific for double-stranded DNA. It has no activity on single-stranded DNA in RT reactions (primers or probes), or on RNA. The enzyme is also thermolabile—it is inactivated quickly at temperatures typical for the SuperScript IV RT reaction (e.g., 50°C). The additional inactivation step is therefore not required in RT-qPCR applications.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Which SuperScript IV RT format do you recommend for real-time PCR applications?

For RT-qPCR applications we recommend using the Invitrogen SuperScript IV VILO Master Mix (Cat. No. 11756050). The cDNA synthesis reaction setup with this master mix requires fewer pipetting steps and therefore reduces variation in the data. SuperScript IV RT, as a component of the master mix, offers the highest efficiency of cDNA synthesis step compared to competitors’ products.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Are there any significant changes in the SuperScript IV RT protocol compared to the SuperScript III RT protocol?

The only change is that the incubation time for the reverse transcription reaction has been reduced from 50 minutes to 10 minutes. All the other parameters and steps are the same.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I get comparable cDNA yield and length using the SuperScript IV RT 10-minute protocol as when using the 50-minute protocol for SuperScript III RT?

When compared with SuperScript III RT (and other manufacturers’ RTs) in a synthesis reaction for a 9 kb cDNA, SuperScript IV RT performed successful synthesis in just 10 minutes and did so with comparable (or improved) yield (as shown by gel band density).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

View all SuperScript™ IV VILO™ Master Mix FAQs