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Gibco™
MEM
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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| Catalog Number | Quantity |
|---|---|
| 11095080 | 500 mL |
| 11095098 | 10 x 500 mL |
| 11095072 | 1000 mL |
| 11095114 | 6 x 1000 mL |
Catalog number 11095080
Price (USD)
34.97
Each
Quantity:
500 mL
Price (USD)
34.97
Each
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
| With | Without |
| • L-glutamine | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using MEM
MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeMEM (Minimum Essential Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesLow Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture