Knockout™ Serum Replacement (Knockout™ SR) is for serum-free, feeder-based culture of embryonic stem cells (ESCs) and induced Pluripotent stem cellsRead more
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Catalog Number
Quantity
10828028
500 mL
10828010
100 mL
2 Options
Catalog number 10828028
Price (USD)
526.00
Each
Add to cart
Quantity:
500 mL
Price (USD)
526.00
Each
Add to cart
Knockout™ Serum Replacement (Knockout™ SR) is for serum-free, feeder-based culture of embryonic stem cells (ESCs) and induced Pluripotent stem cells (iPSCs) from multiple species. This defined, serum-free formulation directly replaces FBS in existing protocols.
Many of the drawbacks of using FBS in stem cell culture, (differentiation of cells and heat-inactivation) can be alleviated with the use of KnockOut™ Serum Replacement. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission should not be compromised. With over 2000 peer-reviewed publications covering applications such as stem cell culture, differentiation, iPSC generation, ESC derivation, and transgenic modeling, KnockOut™ Serum Replacement is widely cited for stem cell culture.
Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
For Use With (Application)Clinical Research, Translational Research
Green FeaturesSustainable packaging
Product LineKnockOut™
pH6 to 8
Shelf Life18 Months
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.
Frequently asked questions (FAQs)
Can KnockOut SR be used to culture mouse ESCs in feeder-free conditions?
Yes, mouse embryonic stem cells can be cultured using KnockOut SR under feeder-free conditions. Typically, cells are plated at a higher seeding density, in the presence of LIF, on a 0.1% gelatin layer in place of feeder cells.