1 Kb Plus DNA Ladder
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1 Kb Plus DNA Ladder
Invitrogen™

1 Kb Plus DNA Ladder

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Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range ofRead more
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Catalog NumberQuantity
107870181 μL DNA Ladder (500 ng), 1 μL Sample Loading Buffer, 500 Applications
107870264 x 500 μL DNA Ladder, 4 x 1 mL Sample Loading Buffer, 2000 Applications
Catalog number 10787018
Price (USD)
206.00
Each
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Quantity:
1 μL DNA Ladder (500 ng), 1 μL Sample Loading Buffer, 500 Applications
Request bulk or custom format
Price (USD)
206.00
Each
Add to cart
Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels.

Highlights of 1 Kb Plus DNA Ladder:
• Sharp, clear bands—chromatography purified fragments for consistent and reliable results
• Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration
• Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.5 μg/μL
Gel TypeAgarose
Product TypeDNA Ladder
Ready to LoadNo
Sample Loading Volume1 mL
Size Range100 to 15000 bp
Volume (Metric)500 μL
Gel CompatibilityAgarose gel
Green FeaturesSustainable packaging
No. of Reactions500 Applications
Quantity1 μL DNA Ladder (500 ng), 1 μL Sample Loading Buffer, 500 Applications
Shipping ConditionApproved for shipment at Room Temperature or on Dry Ice
TechnologyIndividual chromatography-purified DNA fragments
Unit SizeEach
Contents & Storage
• 500 µL 1 Kb Plus DNA Ladder
• 1 mL 10X BlueJuice Gel Loading Buffer

Store at -20°C.

Frequently asked questions (FAQs)

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?

Invitrogen DNA ladders are composed of double-stranded DNA fragments only.

Why are the DNA bands from my molecular weight ladder smearing?

Here are a few reasons why you might see smearing of the bands:

- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.

I'm seeing anomalous migration of my DNA ladder. What happened?

This can happen if the marker was heated. Please ensure that the ladders are not heated before use.

View all 1 Kb Plus DNA Ladder FAQs