Defined Keratinocyte SFM (1X)
Defined Keratinocyte SFM (1X)
Gibco™

Defined Keratinocyte SFM (1X)

Defined Keratinocyte SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need forRead more
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Catalog number 10744019
Price (USD)
150.00
Each
Add to cart
Price (USD)
150.00
Each
Add to cart
Defined Keratinocyte SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers. The growth-promoting activity of BPE has been replaced by the inclusion of growth-promoting agents, which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM), allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes.

Defined Keratinocyte SFM is a complete serum free medium consisting of Defined Keratinocyte Serum Free Basal Medium with the addition of Defined Keratinocyte SFM Growth Supplement that eliminates the requirement for bovine pituitary extract. The supplement is supplied in separate packaging with the basal medium.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeKeratinocyte Cells, Epithelial Cells
ClassificationSerum-free
Concentration1 X
Culture TypeMammalian Cell Culture
FormLiquid
Product TypeKit
Serum LevelSerum-free
Shelf Life12 Months
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red
Green FeaturesSustainable packaging
Quantity500 mL
Unit SizeEach
Contents & Storage
• 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light
• 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5 to -20°C) and protect from light

Complete Medium: store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Are the Defined Keratinocyte SFM basal medium and supplement available separately?

The Defined Keratinocyte SFM basal medium and supplement are not available separately. The kit can be ordered with catalog #10744-019.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.